The Anti-Inflammatory Activity of HMGB1 A Box Is Enhanced When Fused with C-Terminal Acidic Tail
Figure 2
Western blot and SDS-PAGE analysis of the C tail-fused A box proteins. (a) Identification of the two fusion proteins expressed in E. coli DH5 by western blot using a mouse anti-human HMGB1 monoclonal antibody directed against A box. Lane 1:A boxC tail; Lane 2:A box(Gly4Ser)3C tail. (b) Purity analysis of the two fusion proteins by SDS-PAGE. After purification by Ni2+-NTA chromatography, the purities of the two fusion proteins were analyzed with SDS-PAGE. Equal amounts of purity proteins (10 g) were loaded in Figure 2(b), separated by 12% PAGE gel electrophoresis. Lane 1:Molecular weight standards; Lane 2:A boxC tail; Lane 3:A box(Gly4Ser)3C tail.