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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 185683, 8 pages
Research Article

Cardiac Imaging Using Clinical 1.5 T MRI Scanners in a Murine Ischemia/Reperfusion Model

1Department of Internal Medicine III (Cardiology), Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck, Austria
2Department of Radiology I, Innsbruck Medical University, Anichstr. 35, 6020 Innsbruck, Austria

Received 13 July 2010; Accepted 26 October 2010

Academic Editor: Andrea Vecchione

Copyright © 2011 Jakob G. J. Voelkl et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Additional files are presented to ease setup of clinical scanners for cardiac imaging in mice and establishing the described protocol. Positioning of the coil and the ECG on the mouse should be done as to provide the smallest amount of stress for the animal. In this study a trimmed PE Tube was used to enable easy handling of the sedated mouse while mainting a natural posture of the animal. Three differently angled views of the mouse heart were used to provide localization of measurements. This allows exact slice positioning and standardization of measurements.

Supplementary material is also provided to further demonstrate the versatility of clinical MRI scanners in comparison to echocardiography. These additional measurements not described in the results of the manuscript can be of value for specific questions during studies on the mouse heart. Long axis view can be used in addition to short axis view for a more detailed assessment of cardiac function. A clinical scanner is sufficient for an approximate measurement of cardiac ejection fraction and other parameters. The fast low angle shot (FLASH) 3D sequence enables thin slice thickness for a more accurate assessment of infarct size, but is sensible to motion artifacts. A cardiodepressive sedative used in combination with this sequence could facilitate a more exact measurement of infarct size, but will hinder measurement of functional parameters.

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