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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 252141, 10 pages
Methodology Report

Assessment of Elastase-Induced Murine Abdominal Aortic Aneurysms: Comparison of Ultrasound Imaging with In Situ Video Microscopy

1Division of Cardiovascular Medicine, Stanford University school of Medicine, CA, USA
2Department of Vascular Surgery, Stanford University school of Medicine, CA, USA
3Stanford University school of Medicine, 300 Pasteur Drive, Stanford, CA 94305-5406, USA

Received 15 September 2010; Revised 1 December 2010; Accepted 20 December 2010

Academic Editor: Monica Fedele

Copyright © 2011 Junya Azuma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aims. The aim of this study was to definitively assess the validity of noninvasive high-frequency ultrasound (US) measurements of aortic luminal diameter (ALD) in a murine model of elastase-induced abdominal aortic aneurysm in comparison with in situ video microscopy (VM). Methods. C57BL/6 mice underwent transient perfusion of the aorta with either elastase (n=20: Elastase group) or saline (n=10: Sham). Unoperated mice (n=10) were also studied. Results. ALD measurements by US had excellent linear correlation and absolute agreement with that by VM in both Control (unoperated or sham-operated mice) and elastase groups (r=0.96, intraclass correlation coefficient (ICC)=0.88 and r=0.93, ICC=0.92, resp.). Bland-Altman analysis of US compared with VM measurements in both groups indicated good agreement, however US measurements were slightly but significantly higher than VM measurements in the control group (mean bias 0.039 mm, P<.05). Linear regression analysis revealed excellent correlation between US and VM measurements in both groups. (R2=0.91 in Control group, R2=0.85 in elastase group.) The reliability of US measurements was also confirmed by ex vivo histological measurements. Conclusions. High-frequency US provides reliable ALD measurements in developing murine abdominal aortic aneurysms.