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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 502057, 8 pages
http://dx.doi.org/10.1155/2011/502057
Research Article

Orange Juice and Its Component, Hesperidin, Decrease the Expression of Multidrug Resistance-Associated Protein 2 in Rat Small Intestine and Liver

1Department of Pharmacology, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae-Ku, Kawasaki, Kanagawa 216-8511, Japan
2Institution for Animal Experimentation, St. Marianna University Graduate School of Medicine, Kawasaki, Kanagawa 216-8511, Japan
3Department of Pharmacogenomics, St. Marianna University Graduate School of Medicine, Kawasaki, Kanagawa 216-8511, Japan
4Unit of Medical Statistics, Faculty of Medical Education and Culture, St. Marianna University School of Medicine, Kawasaki, Kanagawa 216-8511, Japan

Received 13 September 2010; Revised 25 February 2011; Accepted 24 March 2011

Academic Editor: Phillip E. Klebba

Copyright © 2011 Minoru Watanabe et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the effects of orange juice (OJ) or hesperidin, a component of OJ, on the pharmacokinetics of pravastatin (PRV) and the expression of both protein and mRNA of multidrug resistance-associated protein 2 (Mrp2) in the rat small intestine and liver. Eight-week-old male Sprague-Dawley rats were used in this study. OJ or a 0.079% hesperidin suspension was administered orally for 2 days. Tap water was given as a control. A single dose of PRV at 100 mg/kg p.o. was administered after 2 days of OJ, hesperidin, or tap water ingestion. The AUC, 𝐶 m a x , and t 1/2 values of PRV were significantly increased in OJ group. Mrp2 protein and mRNA levels in the small intestine and liver, respectively, were significantly decreased after the ingestion of OJ. The same results were obtained with hesperidin. These results suggest that the changes in PRV pharmacokinetic parameters and the decrease in Mrp2 expression caused by OJ are due to hesperidin in the juice.