In Vitro Culture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota

Table 2

The identification of the excised DGGE bands obtained from fecal samples of individual A in different medium at zero time and after 18 hours. The position of each band in gel profile is shown in Figure 1(a). The numbers in parentheses refer to GenBank accession number.

Band	Phylum	The closest type strain^a	Similarity	The nearest sequence^b	Similarity
1	Bacteroidetes	Parabacteroides merdae ATCC 43184 (AAXE02000112)	100%	Uncultured bacterium clone HFV09_495 (GU107934)	100%
2	Firmicutes	Ruminococcus torques ATCC 27756 (AAVP02000040)	99.34%	Uncultured bacterium clone 429C08 (HQ237429)	100%
3	Firmicutes	Ruminococcus lactaris ATCC 29176 (L76602)	98.61%	Uncultured bacterium clone S2-190 (GQ898449)	100%
4	Bacteroidetes	Alistipes finegoldii ANH 2437 (AJ518874)	90.18%	Uncultured bacterium clone (FP085183)	98.80%
5(1)	Bacteroidetes	Bacteroides uniformis JCM 5828 (AB050110)	100%	Uncultured bacterium clone 425D06 (HQ237168)	100%
5(2)	Firmicutes	Pseudobutyrivibrio ruminis (X95893)	100%	Uncultured bacterium clone 426B02 (HQ237240)	100%
6	Bacteroidetes	Butyricimonas virosa MT12 (AB443949)	93.33%	Uncultured bacterium clone HFV09_122 (GU107561)	100%
7	Firmicutes	Blautia luti DSM 14534 (AJ133124)	100%	Uncultured bacterium clone 428B12 (HQ237293)	100%
8	Firmicutes	Blautia wexlerae WAL 14507 (EF036467)	99.31%	Uncultured bacterium clone HFV08_232 (GU107126)	99.31%
9	Firmicutes	Subdoligranulum variabile DSM 15176	98.04%	Uncultured bacterium clone HFV08_426 (GU107320)	100%
10	Firmicutes	Phascolarctobacterium faecium ACM3679 (X72865)	100%	Uncultured bacterium clone BFV04_210 (GU099903)	100%
11	Bacteroidetes	Alistipes putredinis DSM 17216 (ABFK01000017)	98.18%	Uncultured bacterium clone HFV09_155 (GU107594)	100%
12	Firmicutes	Ruminococcus flavefaciens ATCC 19208 (X83430)	93.10%	Uncultured bacterium clone HFV07_265 (GU106646)	100%
13	Firmicutes	Faecalibacterium prausnitzii ATCC 27768 (AJ413954)	98.69%	Uncultured bacterium clone b_007_f03 (GQ179311)	100%
14	Firmicutes	Eubacterium tenue ATCC 25553 (DQ445863)	100%	Uncultured bacterium clone 429B01 (HQ237439)	100%
15	Firmicutes	Clostridium quinii DSM 6736 (X76745)	93.91%	Uncultured bacterium clone 429C11 (HQ237448)	100%
16	Firmicutes	Eubacterium eligens ATCC 27750 (CP001104)	98.68%	Uncultured bacterium clone 426G01 (HQ237235)	100%
17	Actinobacteria	Collinsella aerofaciens ATCC 25986 (AAVN02000013)	97.40%	Uncultured bacterium clone RL307_aam06g08 (DQ807453)	99%

^aThe closest type strain information obtained from EzTaxon database.
^bThe nearest sequence was identified using GenBank database.