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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 896162, 5 pages
Research Article

Effect of Colic Vein Ligature in Rats with Loperamide-Induced Constipation

1Department of General Surgery and Transplantation, S. Orsola-Malpighi Hospital, University of Bologna, 40126 Bologna, Italy
2Center for Applied Biomedical Research (CRBA), S. Orsola-Malpighi Hospital, University of Bologna, 40126 Bologna, Italy

Received 29 November 2011; Revised 6 February 2012; Accepted 20 February 2012

Academic Editor: Monica Fedele

Copyright © 2012 Flavia Neri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. Medical treatment in chronic constipation is not always successful. Surgery is indicated in unresponsive selected severe cases. This study presents the distal venous colic ligation in rat as a novel surgical approach. Materials and Methods. 16 rats (study group) were evaluated in 3 phases of 6 days each: A (normal conditions), B (loperamide-induced constipation), and C (colic vein legation) and compared with rats treated in phase C with PEG 4,000 (control group). Blood biochemical and physiological parameters, daily fecal water content (FWC), and histological analysis were performed in all study phases. Results. No biochemical and physiological parameters changes were observed. FWC decreased in phase B and increased in phase C in both groups with a grow up to 2.3-fold in study group compared to control ( 𝑃 < 0 . 0 0 0 1 ). Moreover, in study group, a high number of colonic goblet cells were detected (phase C versus phase B: 𝑃 < 0 . 0 0 1 ) while no differences were registered in control. Conclusion. By ligature of the colic vein in constipated rats, an increase in FWC and goblet cells higher than in PEG treated rats was detected. The described surgical procedure appeared effective, simple, and safe; further studies in animal models, however, are necessary to assess its clinical applicability.