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BioMed Research International
Volume 2013 (2013), Article ID 270898, 7 pages
Research Article

Bovine Papillomavirus in Brazil: Detection of Coinfection of Unusual Types by a PCR-RFLP Method

1Laboratório de Genética, Instituto Butantan, Secretária de Estado da Saúde, Avenida Vital Brasil 1500, Butantan, 05503-900 São Paulo, SP, Brazil
2Laboratório de Biologia Molecular Aplicada e Sorologia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária, e Zootecnia da Universidade de São Paulo, Avenida Prof. Dr. Orlando Marques de Paiva, 87 Cidade Universitária, Butantan, 05508-270 São Paulo, SP, Brazil
3Centro de Pesquisa em Pecuária do Leite, Instituto de Zootecnia, Agencia Paulista de Tecnologia dos Agronegócios, CAPTA Bovinos Leiteiros, Rodovia Luiz de Queiroz km 129, 13460-000 Nova Odessa, SP, Brazil
4Programa de Pós-Graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas, Edifício ICB-III, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 2415 Cidade Universitária, Butantan, 05508-900 São Paulo, SP, Brazil
5Programa de Pós-Graduação em Biologia Estrutural e Funcional, Universidade Federal de São Paulo, Rua Botucatu, 740 Vila Clementino, 04023-900 São Paulo, SP, Brazil
6Departamento de Biologia, Universidade Federal da Integração Latino-Americana (UNILA), Avenida Tancredo Neves 6731, Bloco 4, 85867-970 Foz do Iguaçú, PR, Brazil

Received 26 March 2013; Accepted 16 May 2013

Academic Editor: Lubna Nasir

Copyright © 2013 R. F. Carvalho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bovine papillomavirus (BPV) is recognized as a causal agent of benign and malignant tumors in cattle. Thirteen types of BPV are currently characterized and classified into three distinct genera, associated with different pathological outcomes. The described BPV types as well as other putative ones have been demonstrated by molecular biology methods, mainly by the employment of degenerated PCR primers. Specifically, divergences in the nucleotide sequence of the L1 gene are useful for the identification and classification of new papillomavirus types. On the present work, a method based on the PCR-RFLP technique and DNA sequencing was evaluated as a screening tool, allowing for the detection of two relatively rare types of BPV in lesions samples from a six-year-old Holstein dairy cow, chronically affected with cutaneous papillomatosis. These findings point to the dissemination of BPVs with unclear pathogenic potential, since two relatively rare, new described BPV types, which were first characterized in Japan, were also detected in Brazil.