BioMed Research International / 2013 / Article / Fig 4

Research Article

DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγ/NFκB Pathway in C2C12 Myotubes

Figure 4

Transfection of Stealth RNAi for PPARγ knockdown in C2C12 myotubes. The C2C12 myotubes transfected with either negative control Stealth RNAi oligonucleotide or PPARγ Stealth RNAi oligonucleotide were incubated for 48 h, respectively. Protein extracts from C2C12 myotubes were assayed for western blot analysis with PPARγ (Figure 4(a)). The band on the western blot represented a protein with a molecular mass of ~55 kDa as determined by the molecular mass markers included in the experiment. PPARγ protein expression was determined by western blot and relative abundance of protein was calculated after normalization to β-actin (Figure 4(b)). The PPARγ mRNA was determined using real-time PCR analysis and relative abundance of mRNA was calculated after normalization to β-actin (Figure 4(c)). Data are expressed as mean ± S.D. of 3 different experiments. ** versus positive control group. PPARγ = peroxisome proliferator-activated receptor γ.
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