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BioMed Research International
Volume 2013 (2013), Article ID 425646, 6 pages
http://dx.doi.org/10.1155/2013/425646
Research Article

Detection of Bovine Leukemia Virus in Brains of Cattle with a Neurological Syndrome: Pathological and Molecular Studies

1Pós-Graduação em Sanidade, Segurança Alimentar e Ambiental no Agronegócio, Instituto Biológico de São Paulo (IB), Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil
2Laboratório de Viroses de Bovídeos, Centro de P & D de Sanidade Animal (CPDSA), IB, Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil
3Laboratório de Raiva e Encefalites, CPDSA, IB, Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil
4Laboratório de Bioquímica Fitopatológica, Centro de Sanidade Vegetal, IB, Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil
5Laboratório de Biologia Molecular e Sorologia Aplicadas, Departamento de Medicina Veterinária Preventiva e Saúde Animal, VPS, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Avenida Professor Dr. Orlando Marques de Paiva 87, Cidade Universitária, 05508-270 São Paulo, SP, Brazil
6Laboratório de Anatomia Patológica, CPDSA, IB, Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil

Received 26 December 2012; Revised 8 March 2013; Accepted 12 March 2013

Academic Editor: Lubna Nasir

Copyright © 2013 Rubens Henrique Ramos D’Angelino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME ( ). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions.