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BioMed Research International
Volume 2013 (2013), Article ID 814635, 9 pages
Research Article

Bovine Papillomavirus Type 2 Infection and a Series of Mesenchymal Tumors of the Urinary Bladder in Cattle

1Department of Veterinary Medicine and Animal Productions, Naples University Federico II, Via F. Delpino, 80137 Naples, Italy
2Department of Biology, Naples University Federico II, Via Mezzocannone 16, 80134 Naples, Italy
3Instituto Butantan, Laboratório de Genética, Avenida Vital Brazil 1500, 05503-900 São Paulo, Brazil
4Department of Biopathological Sciences and Hygiene of Animal and Alimentary Productions, University of Perugia, Faculty of Veterinary Medicine, Via San Costanza, 4-06126 Perugia, Italy

Received 28 March 2013; Accepted 16 May 2013

Academic Editor: Lubna Nasir

Copyright © 2013 Manuela Martano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This report describes the histopathology of two hundred and fifty-three mesenchymal tumors of the urinary bladder in cattle grazing on lands rich in bracken fern. Approximately 80% were hemangiomas and angiosarcomas. Hemangioma (capillary, cavernous, and large vessels) was the most frequent mesenchymal tumor and was more common than angiosarcoma. Although the appearance of endothelial cells can vary remarkably, epithelioid angiosarcomas, often containing multinucleated cells, were the most frequent malignant vascular tumors. Hemangiopericytoma and tumors of muscle and soft connective tissue origin, alone and/or in association with tumor-like lesions, were less frequently seen. Furthermore, forty-five cases of intravascular papillary endothelial hyperplasia (IPEH), a lesion not previously reported in the urinary bladder of cattle, were also described. Bovine papillomavirus type-2 DNA was amplified in tumor samples. Forty vascular tumors were investigated by dual-labeling immunofluorescence, and, for the first time, a coexpression of E5 and platelet-derived growth factor β receptor (PDGFβR) was shown to occur. The results show that the BPV-2 E5 oncoprotein binds to the activated form of the PDGFβ receptor thus playing an important role in mesenchymal as well as epithelial carcinogenesis of the urinary bladder. Furthermore, these findings demonstrate that BPV-2 infects both epithelial and mesenchymal cells.