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BioMed Research International
Volume 2014 (2014), Article ID 123482, 9 pages
http://dx.doi.org/10.1155/2014/123482
Research Article

Phosphoproteomic Analysis of Gossypol-Induced Apoptosis in Ovarian Cancer Cell Line, HOC1a

1The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China
2School of Life Sciences, Tsinghua University, Beijing 100084, China
3Peking University People’s Hospital, Beijing 100044, China
4The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China
5Institute of Reproductive Biomedicine, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China

Received 18 May 2014; Revised 18 July 2014; Accepted 20 July 2014; Published 12 August 2014

Academic Editor: Haiteng Deng

Copyright © 2014 Lixu Jin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Ovarian cancer is a major cause for death of gynecological cancer patients. The efficacy of traditional surgery and chemotherapy is rather compromised and platinum-resistant cancer recurs. Finding new therapeutic targets is urgently needed to increase the survival rate and to improve life quality of patients with ovarian cancer. In the present work, phosphoproteomic analysis was carried out on untreated and gossypol-treated ovarian cancer cell line, HOC1a. We identified approximately 9750 phosphopeptides from 3030 phosphoproteins, which are involved in diverse cellular processes including cytoskeletal organization, RNA and nucleotide binding, and cell cycle regulation. Upon gossypol treatment, changes in phosphorylation of twenty-nine proteins including YAP1 and AKAP12 were characterized. Western blotting and qPCR analysis were used to determine expression levels of proteins in YAP1-related Hippo pathway showing that gossypol induced upregulation of LATS1, which phosphorylates YAP1 at Ser 61. Furthermore, our data showed that gossypol targets the actin cytoskeletal organization through mediating phosphorylation states of actin-binding proteins. Taken together, our data provide valuable information to understand effects of gossypol on protein phosphorylation and apoptosis of ovarian cancer cells.