Effect of Angiotensin II and Small GTPase Ras Signaling Pathway Inhibition on Early Renal Changes in a Murine Model of Obstructive Nephropathy

Figure 1

Effect of systemically administrated angiotensin II (Ang II) on Ras signaling pathway. Ras activation was evaluated as Ras GTP by ELISA (a). Phosphorylated (p)-ERK1/2 and p-Akt protein expression were evaluated as the ratio p/total protein by Western blot ((b) and (c), resp.). Bars represent the mean ± SEM of the optical density measured in kidney samples of control saline group (Ctrl; 𝑛=3) and angiotensin II-treated animals (Ang II, 0.8 mg/kg; 𝑛=3–5/per time point). ^∗𝑃<0.05 versus control group.

(a)

(b)

(c)