Effect of Angiotensin II and Small GTPase Ras Signaling Pathway Inhibition on Early Renal Changes in a Murine Model of Obstructive Nephropathy

Figure 7

Effect of chaetomellic acid A administration on UUO-induced Ras pathway activation and fibrotic changes analyzed by Western blot. Protein expression of pERK1/2 and ERK1/2 (a), pAkt and Akt (b), alpha-smooth muscle actin (α-SMA) (c), and fibronectin (d) was detected by immunoblotting. Bars represent the mean ± SEM of the optical density measured in nonobstructed (NO) and obstructed (O) kidney samples of vehicle (𝑛=4) and chaetomellic acid A-treated animals (Chaet.; 1.5 mg/kg; 𝑛=4). ^∗𝑃<0.01 versus NO vehicle-treated kidneys of UUO mice. ^#𝑃<0.01 versus O vehicle-treated kidneys. ^&𝑃<0.01 versus NO Chaet-treated kidney.

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