Research Article
Potential Probiotic Escherichia coli 16 Harboring the Vitreoscilla Hemoglobin Gene Improves Gastrointestinal Tract Colonization and Ameliorates Carbon Tetrachloride Induced Hepatotoxicity in Rats
Table 1
Bacterial strains and plasmids.
| Plasmids/strains | Relevant characteristics | Reference |
| Plasmids | | | pUC-gfp | Derived from the high-copy number vector pUC18 by insertion of a modified gfp gene; Apr | [26] | pUC8:16 | Derived from the high-copy number vector pUC8 by insertion of a vgb gene; Apr | [19] | pUC8:16-gfp | Derived from the high-copy number vector pUC8:16 by insertion of a gfp gene; Apr | This study | Bacterial strains | | | E. coli DH5α | F-endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15Δ(lacZYA-argF)U169, hsdR17 (rK− mK+), λ− | [24] | E. coli BL21 | F
′
ompT hsdSB (rB− mB−) gal dcm | [24] | E. coli 16 | Wild type | [13] | E. coli 16 (pUC-gfp) | E. coli 16 with pUC-gfp plasmid; Apr | This study | E. coli 16 (pUC8:16-gfp) | E. coli 16 with pUC8:16-gfp plasmid; Apr | This study |
|
|
Apr represents ampicillin resistance; gfp represents green fluorescent protein; pUC8:16-gfp represents construct containing vgb gene tagged with gfp.
|