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BioMed Research International
Volume 2014, Article ID 398108, 6 pages
Research Article

Assessment of the BD MGIT TBc Identification Test for the Detection of Mycobacterium tuberculosis Complex in a Network of Mycobacteriology Laboratories

1Grupo de Micobactérias, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), Rua da Junqueira 100, 1349-008 Lisboa, Portugal
2Centro de Recursos Microbiológicos (CREM), UNL, 2829-516 Caparica, Portugal
3Instituto Nacional de Saúde, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique
4Programa Nacional de Controlo da Tuberculose, Ministério da Saúde de Moçambique, 264 Cidade de Maputo, Mozambique
5Centro de Malária e Outras Doenças Tropicais/LA, IHMT/UNL, Rua da Junqueira 100, 1349-008 Lisboa, Portugal

Received 11 October 2013; Accepted 18 November 2013; Published 23 January 2014

Academic Editor: Tomasz Jagielski

Copyright © 2014 Diana Machado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specificity of 100%, and positive and negative predictive values of 100% and 80.55%, respectively. The test is rapid, easy to perform and interpret, and does not require sample preparation or instrumentation. However, a negative result does not exclude the presence of a strain belonging to MTBC, especially when mutations in mpb64 gene are present or some M. bovis BCG strains are isolated. The TBcID showed potential to assist in the identification of MTBC when the implementation and usage of molecular methods are often not possible, principally in resource-limited countries.