BioMed Research International / 2014 / Article / Tab 2

Research Article

Extracts from Vatica diospyroides Type SS Fruit Show Low Dose Activity against MDA-MB-468 Breast Cancer Cell-Line via Apoptotic Action

Table 2

The acetone and methanol extracts of V. diospyroides type SS fruit at their IC50 concentrations (see Table 1) were inactive on normal Vero cells. The cell growth was assessed by a green fluorescent protein (GFP) assay.

Extract-fruit partExtract concentrations (μg/mL)% Cell growth*ActivityIC50 (μg/mL)

Acetone-cotyledon50.0026.53 ± 11.34bCytotoxic43.90 ± 4.67
16.67102.74 ± 2.56aNoncytotoxicN/A
5.56104.17 ± 14.26aNoncytotoxicN/A
1.85108.71 ± 10.00aNoncytotoxicN/A
0.62107.94 ± 10.79aNoncytotoxicN/A
0.21110.78 ± 12.40aNoncytotoxicN/A

Acetone-pericarp50.00103.49 ± 32.03aNoncytotoxicN/A
16.67114.56 ± 7.96aNoncytotoxicN/A
5.56110.46 ± 11.19aNoncytotoxicN/A
1.85108.13 ± 10.80aNoncytotoxicN/A
0.62110.54 ± 5.94aNoncytotoxicN/A
0.21111.26 ± 6.65aNoncytotoxicN/A

Methanol-cotyledon50.0088.01 ± 15.42aNoncytotoxicN/A
16.67115.46 ± 11.87aNoncytotoxicN/A
5.56112.69 ± 8.84aNoncytotoxicN/A
1.85116.70 ± 11.63aNoncytotoxicN/A
0.62116.26 ± 11.07aNoncytotoxicN/A
0.21117.57 ± 7.79aNoncytotoxicN/A

Methanol-pericarp50.0025.34 ± 15.50bCytotoxic48.75 ± 0.79
16.67112.68 ± 6.99aNoncytotoxicN/A
5.56104.09 ± 6.55aNoncytotoxicN/A
1.85104.45 ± 11.47aNoncytotoxicN/A
0.62104.64 ± 6.67aNoncytotoxicN/A
0.21104.89 ± 9.67aNoncytotoxicN/A

N/A means not applicable IC50 calculation.
-*Cell growth reduced to less than 50% would indicate cytotoxicity against the Vero cells; otherwise an extract was considered inactive and the IC50 level could not be determined (N/A). Three independent replicates were performed.
-Different superscripts within a column indicate statistically significant differences in the Vero cell growth (mean ± SD) according to Tukey’s multiple comparison test.

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