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BioMed Research International
Volume 2014, Article ID 483596, 10 pages
http://dx.doi.org/10.1155/2014/483596
Research Article

Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method

Department of Chemical Engineering and Materials Science, University of California at Davis, 1 Shields Avenue, Davis, CA 95616, USA

Received 28 November 2013; Revised 4 April 2014; Accepted 29 April 2014; Published 26 May 2014

Academic Editor: Qiang “Shawn” Chen

Copyright © 2014 Nathaniel J. Kingsbury and Karen A. McDonald. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [10 citations]

The following is the list of published articles that have cited the current article.

  • J.F. Buyel, R.M. Twyman, and R. Fischer, “Extraction and downstream processing of plant-derived recombinant proteins,” Biotechnology Advances, 2015. View at Publisher · View at Google Scholar
  • Naomichi Fujiuchi, Ryo Matsuda, Nobuyuki Matoba, and Kazuhiro Fujiwara, “ Removal of bacterial suspension water occupying the intercellular space of detached leaves after agroinfiltration improves the yield of recombinant hemagglutinin in a Nicotiana benthamiana transient gene expression system ,” Biotechnology and Bioengineering, 2015. View at Publisher · View at Google Scholar
  • Johannes F. Buyel, “Process Development Strategies in Plant Molecular Farming,” Current Pharmaceutical Biotechnology, vol. 16, no. 11, pp. 966–982, 2015. View at Publisher · View at Google Scholar
  • Jürgen Hubbuch, Rainer Fischer, and Johannes F. Buyel, “Comparison of tobacco host cell protein removal methods by blanching intact plants or by heat treatment of extracts,” Journal of Visualized Experiments, vol. 2016, no. 114, 2016. View at Publisher · View at Google Scholar
  • Ewelina Łojewska, Tomasz Kowalczyk, Szymon Olejniczak, and Tomasz Sakowicz, “Extraction and purification methods in downstream processing of plant-based recombinant proteins,” Protein Expression and Purification, vol. 120, pp. 110–117, 2016. View at Publisher · View at Google Scholar
  • Aparajita Chatterjee, Narayan C. Das, Sumita Raha, Indu B. Maiti, Ankita Shrestha, Ahamed Khan, Sefali Acharya, and Nrisingha Dey, “Enrichment of apoplastic fluid with therapeutic recombinant protein for efficient biofarming,” Biotechnology Progress, 2017. View at Publisher · View at Google Scholar
  • Zivko L. Nikolov, Jeffrey T. Regan, Lynn F. Dickey, and Susan L. Woodard, “Purification Of Monoclonal Antibodies From Plants,” Process Scale Purification of Antibodies, pp. 631–653, 2017. View at Publisher · View at Google Scholar
  • Johannes F. Buyel, Richard M. Twyman, and Rainer Fischer, “Very-Large-Scale Production Of Monoclonal Antibodies In Plants,” Process Scale Purification of Antibodies, pp. 655–672, 2017. View at Publisher · View at Google Scholar
  • Kalimuthu Karuppanan, Sifti Duhra-Gill, Muchena Kailemia, My Phu, Carlito Lebrilla, Abhaya Dandekar, Raymond Rodriguez, Somen Nandi, and Karen McDonald, “Expression, Purification, and Biophysical Characterization of a Secreted Anthrax Decoy Fusion Protein in Nicotiana benthamiana,” International Journal of Molecular Sciences, vol. 18, no. 1, pp. 89, 2017. View at Publisher · View at Google Scholar
  • Yongao Xiong, Qiongyu Li, Muchena Kailemia, Carlito Lebrilla, Somen Nandi, and Karen McDonald, “Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration,” International Journal of Molecular Sciences, vol. 19, no. 3, pp. 890, 2018. View at Publisher · View at Google Scholar