Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris

Figure 6

CvHSP90 mRNA expression levels relative to β-actin mRNA levels under stress of different salt concentrations analyzed by real-time quantitative RT-PCR. The β-actin gene was used as an internal control to calibrate the cDNA template for all the samples. Vertical bars represented the mean ± SE (𝑁=5). Significant differences across control were indicated with an asterisk at 𝑃<0.05 and two asterisks at 𝑃<0.01.