L-Methionase: A Therapeutic Enzyme to Treat Malignancies

Figure 3

Methionase qualitative detection by DTNB. Methionase catalysis conversion of L-methionine as a substrate in agar plates into α-ketobutyrate, ammonia, and methanethiol. DTNB reagent reacts with SH (thiol) functional group and gives intense yellow coloration around methionase producing bacterial isolates.