-Mangostin Suppresses the Viability and Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells by Downregulating the PI3K/Akt Pathway
α-Mangostin induces apoptosis of pancreatic cancer cells. (a) Panc-1 and BxPC-3 cells were treated with α-mangostin (0 μM, 8 μM, or 16 μM) for 24 h. Apoptotic cells were evaluated by Annexin V-FITC/PI staining and flow cytometry. Representative FACS plots are shown. (b) Apoptosis rate of the early and late apoptosis was quantified by flow cytometry. compared with controls. (c) Protein levels of Bcl-2 and cleaved caspase-3 were detected by Western blotting with β-actin as loading control.