-Mangostin Suppresses the Viability and Epithelial-Mesenchymal Transition of Pancreatic Cancer Cells by Downregulating the PI3K/Akt Pathway
Figure 6
α-Mangostin suppresses activation of the PI3K/Akt pathway in pancreatic cancer cells. Protein levels of total Akt and pAkt-S473 were detected by Western blotting. (a) Panc-1 and BxPC-3 cells were treated with α-mangostin (16 μM) for 0 h, 3 h, 6 h, 12 h, and 24 h. (b) Panc-1 and BxPC-3 cells were treated with α-mangostin at the indicated concentrations for 24 h. (c) Panc-1 and BxPC-3 cells were treated with 5 ng/mL TGF-β either alone or in combination with 16 μM α-mangostin for 24 h. Protein levels of total Akt, pAkt-S473, E-cadherin, vimentin, and N-cadherin were measured by Western blotting with β-actin as loading control.
