Saccharin Sulfonamides as Inhibitors of Carbonic Anhydrases I, II, VII, XII, and XIII
Figure 3
The FTSA dosing curves of compounds 1 (saccharin, panel (a)) and 4 (b) binding to CAs I, II, VII, XII, and XIII. Saccharin was dosed up to 7.5 mM and a small shift was observed for all CAs except CA I. Compound 4 was dosed up to 200 μM with a significant shift. The determined dissociation constants for all compounds are listed in Table 1. There was no added DMSO while dosing saccharin and there was 2% (v/v) final DMSO concentration while dosing 4. This explains the reduced of the protein in the absence of compound with DMSO (b) compared to (a) that in the absence of DMSO.
