Identification of a Novel Methylated Gene in Nasopharyngeal Carcinoma: TTC40

Table 2

Summary of the two major differentially methylated DNA fragments identified by MS/AP-PCR.

Fragment	Size (bp)	CpG dinucleotide	CpG island^a	Unique/repetitive	Chromosome map
Hypermethylated
Sequence-1	334	25	Yes	Unique	10q26.3
Sequence-2	336	3	No	Unique	1p13.3
Hypomethylated
Sequence-1	400	7	No	Repetitive (6x)	16p11.1
Sequence-2^b	406	8	No	Repetitive (6x)	16p13.1

aThe presence of CpG island was determined based on the following criteria: minimum length, 200 bp; CG content, >50%; Obs/Exp, >0.6.
^bThat is different from sequence-1 by the insertion of 6 bp.