BioMed Research International / 2014 / Article / Fig 6

Research Article

N-acetyl-seryl-aspartyl-lysyl-proline Inhibits Diabetes-Associated Kidney Fibrosis and Endothelial-Mesenchymal Transition

Figure 6

Antifibrotic effect of AcSDKP is associated with restoration of FGFR levels. (a) Western blot analysis of FGF receptor phosphorylation and total protein levels are shown. Actin is shown as a loading control. ((b)-(c)) Densitometric analysis of phospho-FGF receptor and total FGF receptor levels normalized to actin. The results are shown as the relative expression against the control animal values. The data are shown as the mean ± SEM values ( ). (d) qPCR analysis for FGF receptor in kidney. The data are shown as the mean ± SEM values. in each group were analyzed. (e) Western blot analysis for FGF receptor levels and its phosphorylation. The same sample as Figure 5(d) was analyzed for FGF-R status. Representative results ( ) were shown. ((f), (g)) Densitometric analysis of phospho-FGF receptor and total FGF receptor levels normalized to actin. The results are shown as the relative expression against the control value. The data are shown as the mean ± SEM values ( ). TGF- receptor I on endothelial cells in diabetic mice. ((h)–(k)). Immunolabeling for TGF- receptor I (T RI) and CD31 in a kidney from indicated group of mice. Arrows indicate cells that are double-labeled for T RI and CD31. Scale bar: 25 μm. (l) Quantification of cells expressing T RI and CD31. Ratio of T RI positive on all CD31 positive cells in each visual field among 4 images from one animal using fluorescence microscopy and quantified. The data are expressed as the mean ± SEM values. Diabetes is designated as DM. For all of the groups mice were analyzed.
696475.fig.006a
(a)
696475.fig.006b
(b)
696475.fig.006c
(c)
696475.fig.006d
(d)
696475.fig.006e
(e)
696475.fig.006f
(f)
696475.fig.006g
(g)
696475.fig.006h
(h)
696475.fig.006i
(i)
696475.fig.006j
(j)
696475.fig.006k
(k)
696475.fig.006l
(l)