Description of a Novel Adhesin of Mycobacterium avium Subsp. paratuberculosis

Table 1

Immunogenic CW proteins of MAP identified by MALDI-TOF MS. Proteins were excised from Coomassie blue stained gels and subsequently identified by MALDI-TOF at the Mass Spectrometry Center for Biological and Chemical Analysis (CEQUIBIEM) at the School of Exact and Natural Sciences, University of Buenos Aires. ND: no data.

Spot number	MAP Locus	Mtb Locus	Gene	Protein function/family	ORF size (bp)	Previously described as envelope protein
1	MAP1164	Rv1436	gapdh	Glyceraldehyde-3-phosphate dehydrogenase	1,020	He and de Buck, 2010 (MAP) [34]
2	MAP1205	Rv1479	moxR	ATPase	1,143	Mawuenyega et al., 2005 (Mtb) [35]
3	MAP1325	Rv1630	rpsA S1	30S ribosomal protein S1	1,443	Gu et al., 2003 (Mtb) [36]
4	MAP1367	Rv1658	argG	Argininosuccinate synthase	1,197	ND
5	MAP1889c	Rv2145c	wag31	DivIVA family protein	783	He and de Buck, 2010 (MAP) [34]
6	MAP1962	Rv2220	glnA1	Glutamine synthetase A1	1,437	Gu et al., 2003 (MAP) [36]
7	MAP1998	Rv2245	kasA	3-Oxoacyl synthetase	1,251	He and de Buck, 2010 (MAP) [34]
8	MAP1999	Rv2246	kasB_1	3-Oxoacyl sintase 2 B	1,323	Mawuenyega et al., 2005 (Mtb) [35]
9	MAP2453c	Rv1308	atpA	ATP synthase subunit alpha	1,665	He and de Buck, 2010 (MAP) [34]
10	MAP2855c	Rv2744c	35kd_ag	Phage shock protein A	828	ND
5	MAP3152c	Rv3075c	hpcH/hpaI	Aldolase/citrate lyase	921	Gu et al., 2003 (MAP) [36]
11	MAP3404	Rv3285	accA3	Carbamoyl-phosphate synthase subunit A	1,824	Mawuenyega et al., 2005 (Mtb) [35]
12	MAP3567	Rv0148	Hypothetical protein	Short-chain dehydrogenases/reductases	864	He and de Buck, 2010 (MAP) [34]
13	MAP3651c	Rv0215c	fadE3_2	Acyl-CoA dehydrogenase FadE3	1,218	He and de Buck, 2010 (MAP) [34]
7	MAP3692c	Rv0242c	fabG4	3-Ketoacyl reductase	1,365	He and de Buck, 2010 (MAP) [34]
14	MAP3853	Rv0384c	clpB	ATP dependent protease ClpB	2,547	He and de Buck, 2010 (MAP) [34]
3	MAP3936	Rv0440	groEL2	GroEL chaperonin	1,626	He and de Buck, 2010 (MAP) [34]
15	MAP4143	Rv0685	eftu	Elongation factor Tu	1,191	He and de Buck, 2010 (MAP) [34]