Lentivirus Mediated siRNA against GluN2B Subunit of NMDA Receptor Reduces Nociception in a Rat Model of Neuropathic Pain

Figure 2

mRNA expression of GluN2B detected by real-time PCR. As shown in Figure 2, CCI increased the GluN2B mRNA expression in Lv-siGluN2B group, Lv-GFP group, and NS group. On the 3rd, 7th, 10th, 14th, 21st, 28th, and 35th days after delivery of Lv-siGluN2B, the GluN2B mRNA expression induced by nerve ligation decreased significantly compared to Lv-GFP group and NS group ( versus NS and Lv-GFP groups, two-way ANOVO analysis followed by Turkey’s multiple comparisons, ). No differences were observed on the 1st day.