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BioMed Research International
Volume 2014, Article ID 892704, 6 pages
Research Article

The Effects of Remote Ischemic Preconditioning and N-Acetylcysteine with Remote Ischemic Preconditioning in Rat Hepatic Ischemia Reperfusion Injury Model

1Department of Anesthesiology and Reanimation, Dokuz Eylul University Medical School, Inciralti, 35340 Izmir, Turkey
2Department of Histology and Embryology, University Medical School, 35340 Izmir, Turkey
3Department of Laboratory Animal Science, University Medical School, 35340 Izmir, Turkey
4Department of Biochemistry, University Medical School, 35340 Izmir, Turkey
5Department of Public Health, University Medical School, 35340 Izmir, Turkey

Received 27 August 2013; Accepted 22 November 2013; Published 8 January 2014

Academic Editor: Ahmet Eroglu

Copyright © 2014 Ali Ihsan Uysal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Remote ischemic preconditioning (RIP) and pharmacological preconditioning are the effective methods that can be used to prevent ischemia reperfusion (IR) injury. The aim of this study was to evaluate the effects of RIP and N-Acetylcysteine (NAC) with RIP in the rat hepatic IR injury model. Materials and Methods. 28 rats were divided into 4 groups. Group I (sham): only laparotomy was performed. Group II (IR): following 30 minutes of hepatic pedicle occlusion, 4 hours of reperfusion was performed. Group III (RIP + IR): following 3 cycles of RIP, hepatic IR was performed. Group IV (RIP + NAC + IR): following RIP and intraperitoneal administration of NAC (150 mg/kg), hepatic IR was performed. All the rats were sacrificed after blood samples were taken for the measurements of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and liver was processed for conventional histopathology. Results. The hepatic histopathological injury scores of RIP + IR and RIP + NAC + IR groups were significantly lower than IR group ( , , resp.). There were no significant differences in AST and ALT values between the IR, RIP + IR, and RIP + NAC + IR groups. Conclusions. In the present study, it was demonstrated histopathologically that RIP and RIP + NAC decreased hepatic IR injury significantly.