Research Article
Absence of Cospeciation between the Uncultured Frankia Microsymbionts and the Disjunct Actinorhizal Coriaria Species
Table 2
Primers used for PCR amplification and DNA sequencing.
| | Gene primers | Sequence (5′-3′) | Amplicons approximate size (bp) | References |
| | glnA | | | | | DB41 | TTCTTCATCCACGACCCG | 500 |
(Clawson et al., 2004 [4]) | | DB44 | GGCTTCGGCATGAAGGT | | dnaA | | | | | F7154_dnaAF | GAGGARTTCACCAACGACTTCAT | 700 | Bautista et al. unpublished | | F7155_dnaAR | CRGAAGTGCTGGCCGATCTT | | IGS nifD-K | | | | | F9372_nifD1 5 | GTCATGCTCGCCGTCGGNG | 700 | This study | | F9374_nifK1 5 | GTTCTTCTCCCGGTAyTCCCA | | F9373_nifD2 5 | ACCGGCTACGAGTTCGCNCA | 700 | This study | | F9375_nifK2 5 | TGCGAGCCGTGCACCAGNG | | 18S-ITS1-5.8S-ITS2-28S | | | | | ITS1 | TCCGTAGGTGAACCTGCGG | 700 |
(White et al., 1990 [52]) | | ITS4 | TCCTCCGCTTATTGATATGC | | F9030-CJ-ITSF | AGCCGGACCCGCGACGAGTTT | 400 | This study | | F9031-CJ-ITSR | CGACGTTGCGTGACGACGCCCA | | matK | | | | | F9249-matKF | ACATTTAAATTATGTGTCAG | 700 | This study | | F9250-matkR | TGCATATACGCACAAATC |
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