Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth
Figure 2
SCIDs and SHEDs showed similar osteo-/dentinogenic differentiation potentials. (a–d) Both SCIDs and SHEDs were cultured with osteo-/dentinogenic differentiation medium. (a) After 7 days, cells were stained with Fast Red Kit (Sigma-Aldrich). (c) ALP activities were similarly enhanced in SCIDs and SHEDs. (b) After 14 days, cells were stained with 2% Alizarin red. (d) Mineralization was similarly enhanced in SCIDs and SHEDs after osteo-/dentinogenic induction. (e, f) Real-time RT-PCR results indicated that dentinogenic makers, (e) DMP-1 and (f) DSPP, were similarly increased in SCIDs and SHEDs. GAPDH was used as an internal control. (g–i) Real-time RT-PCR showed that osteogenic markers, (g) BSP and (h) OCN, were similarly increased in SCIDs and SHEDs. (i) OPN expression was not significantly changed in SCIDs or SHEDs after culturing with osteogenic medium. Student’s -test was performed to determine statistical significance. All error bars represent s.d. (). *. **; NS: no significant difference.
