Research Article

Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Figure 4

Influence of aECM on TNAP activity of osteoblast-precursor cells, premature and mature osteoblasts. Various human (hBMSC, MG-63, SaOS-2) (a), rat (rCaOB, rBMSC) (b), and mouse cell (line)s (MC3T3-E1, MLO-Y4) (c) were plated in BM on aECM composed of collagen I and sCS1, sCS3, HA, and sHA3, respectively. At day 4 after plating cells were cultured either in BM, OM, or OM/D. At day 11 after plating, TNAP activity was determined in cell lysates with p-nitrophenylphosphate as a substrate. The released p-nitrophenolate was measured photometrically at 405 nm. TNAP activity in mU/mL was normalized to protein concentration in mg/mL determined with Rotiquant assay. Data are presented as % of TCPS values in the corresponding cell culture medium. Significant differences of OM/D, OM, and BM on (s) GAG versus col-aECM were calculated by two-way ANOVA and indicated with a ( ), b ( ), and c ( ), .