Artificial Extracellular Matrices with Oversulfated Glycosaminoglycan Derivatives Promote the Differentiation of Osteoblast-Precursor Cells and Premature Osteoblasts

Table 2

Cell culture conditions.

Cells	Species	Expansion medium	Seeding density; [cells/cm²]	Source	Characteristics
hBMSC	Human	DMEM/10% HI-FCS	7,000	Iliac crest/bone marrow	Primary cells	Osteoblast-precursors
MG-63	Human	DMEM/10% HI-FCS	12,500	Long bones/osteosarcoma	Cell line	Premature osteoblasts
SaOS-2	Human	McCoys5A/15% HI-FCS	12,500	Long bones/osteosarcoma	Cell line	Mature osteoblasts
rCaOB	Rat	DMEM/10% HI-FCS	12,500	Skull/calvariae	Primary cells	Premature osteoblasts
rBMSC	Rat	DMEM/10% HI-FCS	12,500	Long bones/bone marrow	Primary cells	Osteoblast-precursors
MC3T3-E1	Mouse	𝛼-MEM/10% HI-FCS	12,500	Skull/calvariae	Cell line	Premature osteoblasts
MLO-Y4	Mouse	𝛼-MEM/10% HI-FCS	12,500	Long bones/femora, tibiae	Cell line	Senescent osteoblasts (osteocytes)