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BioMed Research International
Volume 2014, Article ID 951978, 6 pages
http://dx.doi.org/10.1155/2014/951978
Research Article

Assessment of Mycobacterium bovis Deleted in p27-p55 Virulence Operon as Candidate Vaccine against Tuberculosis in Animal Models

1Instituto de Biotecnología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina
2Public Health England, Porton Down, Salisbury SP4 0JG, UK
3Instituto de Patobiología, CICVyA-INTA, N. Repetto and De los Reseros, 1686 Hurlingham, Argentina

Received 10 October 2013; Revised 21 November 2013; Accepted 28 November 2013; Published 21 January 2014

Academic Editor: Jarosław Dziadek

Copyright © 2014 María V. Bianco et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A Mycobacterium bovis knockout in p27-p55 operon was tested as an antituberculosis experimental vaccine in animal models. The mutant MbΔp27-p55 was significantly more attenuated in nude mice than its parental strain but more virulent than BCG Pasteur. Challenge experiments in mice and guinea pigs using M. bovis or M. tuberculosis strains showed similar protection conferred by MbΔp27-p55 mutant than BCG in terms of pathology and bacterial loads in spleen but lower protection than BCG in lungs. When tested in cattle, MbΔp27-p55 did not induce IL-2 expression and induced a very low production of IFN , suggesting that the lack of P27/P55 reduces the capacity of M. bovis of triggering an adequate Th1 response.