Research Article
Development of Enhanced Primer Sets for Detection of Norovirus
Table 3
The nucleotide sequences of NV GI (a) and NV GII (b) strains were compared with sequences of designed primer sets.
(a) GI |
| NKI primer set | Primer | NKI-F (forward) | | G | T | A | A | A | T | G | A | T | G | A | T | G | G | C | G | T | C | T | A | A | |
| M87661 (GI) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF039732 (GI.1) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF306212 (GI.2) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN603244 (GI.3) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN603245 (GI.4) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JQ388274 (GI.6) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN183161 (GI.7) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF586507 (GI.9) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 |
| Primer | NKI-R (reverse) | | | A | C | C | C | A | D | C | C | A | T | T | R | T | A | C | A | T | Y | T | G | | |
| M87661 (GI) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF039732 (GI.1) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF306212 (GI.2) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN603244 (GI.3) | . | . | . | . | . | G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 1 | JN603245 (GI.4) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JQ388274 (GI.6) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN183161 (GI.7) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF586507 (GI.9) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 |
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(b) GII |
| NKII primer set | Primer | NKII-F (forward) | | C | T | Y | A | G | G | C | A | R | A | T | G | T | A | C | T | G | G | A | C | Y | |
| X86557 (GII) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN797508 (GII.1) | . | . | . | . | . | A | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | 2 | KF429769 (GII.2) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF306213 (GII.3) | . | . | . | . | . | A | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | 2 | JX459907 (GII.4) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KC464499 (GII.12) | . | . | . | . | . | A | . | . | . | . | . | . | . | . | T | . | . | . | . | . | . | 2 | KC597139 (GII.17) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN899245 (GII.21) | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 1 |
| Primer | NKII-R (reverse) | | | T | C | G | A | C | G | C | C | A | T | C | T | T | C | A | T | T | C | A | C | | |
| X86557 (GII) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN797508 (GII.1) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF429769 (GII.2) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KF306213 (GII.3) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JX459907 (GII.4) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KC464499 (GII.12) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | KC597139 (GII.17) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 | JN899245 (GII.21) | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 |
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