Development of Enhanced Primer Sets for Detection of Norovirus

Table 4

Information of NV GI primer sets.

Primers	Sequence (5′-3′)^a	Location^b	Region	Annealing temperature (°C)	Polarity	Reference
NKI-F	GTA AAT GAT GAT GGC GTC TAA	5354–5373	Capsid	51	+	This study
NKI-R	ACC CAD CCA TTR TAC ATY TG	5649–5668			−
NKI-F2	GAT GGC GTC TAA GGA CGC	5363–5380			+
GI-F1	CTG CCC GAA TTY GTA AAT GAT GAT	5342–5365	Capsid	55	+	[26]
GI-R1	CCA ACC CAR CCA TTR TAC ATY TG	5649–5671			−
GI-F2	ATG ATG ATG GCG TCT AAG GAC GC	5358–5380			+
SRI-1	CCA ACC CAR CCA TTR TAC AT	5652–5671	Capsid	50	−	[27]
SRI-2	AAA TGA TGA TGG CGT CTA	5356–5373			+
SRI-3	AAA AYR TCA CCG GGK GTA T	5578–5596			−

The means of alphabet sequence are the following: Y = C, T; R = A, G; K = G, T; D = A, G, T.
^bLocation is based on accession number M87661 (Norwalk virus).