Development of Enhanced Primer Sets for Detection of Norovirus

Table 5

Information of NV GII primer sets.

Primers	Sequence (5′-3′)^a	Location^b	Region	Annealing temperature (°C)	Polarity	Reference
NKII-F	CTY AGG CAR ATG TAC TGG ACY	4805–4825	RdRp	55	+	This study
NKII-R	TCG ACG CCA TCT TCA TTC AC	5081–5100			−
NKII-R2	GGA GCC AGA TTG CGA TCG C	5060–5078			−
GII-F1	GGG AGG GCG ATC GCA ATC T	5049–5067	Capsid	55	+	[26]
GII-R1	CCR CCI GCA TRI CCR TTR TAC AT	5367–5389			−
GII-F2	TTG TGA ATG AAG ATG GCG TCG ART	5079–5102			+
SRII-1	CGC CAT CTT CAT TCA CAA A	5078–5096	RdRp	50	−	[27]
SRII-2	TWC TCY TTY TAT GGT GAT GAT GA	4583–4605			+
SRII-3	TTW CCA AAC CAA CCW GCT G	4767–4785			−

The means of alphabet sequence are the following: Y = C, T; R = A, G; I = inosine; W = A, T.
^bLocation is based on accession number X86557 (Lordsdale virus).