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BioMed Research International
Volume 2015, Article ID 124721, 8 pages
http://dx.doi.org/10.1155/2015/124721
Research Article

Protecting Intestinal Epithelial Cell Number 6 against Fission Neutron Irradiation through NF-κB Signaling Pathway

1Department of Experimental Pathology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District 100850, China
2Department of Medical Oncology, Air Force PLA General Hospital, Fucheng Road No. 30, Haidian District, Beijing 100142, China
3The Neurology Department of the 148th Hospital, 20 Zhanbei Road, Zibo 255300, China

Received 28 June 2014; Accepted 26 July 2014

Academic Editor: Hongwei Wang

Copyright © 2015 Gong-Min Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The purpose of this paper is to explore the change of NF-κB signaling pathway in intestinal epithelial cell induced by fission neutron irradiation and the influence of the PI3K/Akt pathway inhibitor LY294002. Three groups of IEC-6 cell lines were given: control group, neutron irradiation of 4Gy group, and neutron irradiation of 4Gy with LY294002 treatment group. Except the control group, the other groups were irradiated by neutron of 4Gy. LY294002 was given before 24 hours of neutron irradiation. At 6 h and 24 h after neutron irradiation, the morphologic changes, proliferation ability, apoptosis, and necrosis rates of the IEC-6 cell lines were assayed and the changes of NF-κB and PI3K/Akt pathway were detected. At 6 h and 24 h after neutron irradiation of 4Gy, the proliferation ability of the IEC-6 cells decreased and lots of apoptotic and necrotic cells were found. The injuries in LY294002 treatment and neutron irradiation group were more serious than those in control and neutron irradiation groups. The results suggest that IEC-6 cells were obviously damaged and induced serious apoptosis and necrosis by neutron irradiation of 4Gy; the NF-κB signaling pathway in IEC-6 was activated by neutron irradiation which could protect IEC-6 against injury by neutron irradiation; LY294002 could inhibit the activity of IEC-6 cells.