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BioMed Research International
Volume 2015, Article ID 134050, 21 pages
http://dx.doi.org/10.1155/2015/134050
Research Article

Identification of Glioblastoma Phosphotyrosine-Containing Proteins with Two-Dimensional Western Blotting and Tandem Mass Spectrometry

Tianyao Guo,1,2,3 Xiaowei Wang,1,2,3 Maoyu Li,1,2,3 Haiyan Yang,1,2,3 Ling Li,1 Fang Peng,1,2,3 and Xianquan Zhan1,2,3,4

1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China
2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China
3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, China
4The State Key Laboratory of Medical Genetics, Central South University, 88 Xiangya Road, Changsha, Hunan 410008, China

Received 11 June 2014; Revised 25 August 2014; Accepted 25 August 2014

Academic Editor: Fa-Yun Che

Copyright © 2015 Tianyao Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Table 1: Proteins without tyrosine phosphorylated motif, identified with 2DGE-based Western blotting and MS/MS

Supplemental Table 2: Phosphotyrosine-containing proteins identified in a human glioblastoma tissue for IPA analysis

Supplemental Table 3: Signaling pathway networks that involve phosphotyrosine-containing proteins identified from human glioblastoma

Supplemental Figure 1: Significant canonical pathways involved phosphotyrosine-containing proteins in a glioblastoma tissue

  1. Supplementary Material