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BioMed Research International
Volume 2015, Article ID 162439, 10 pages
Research Article

The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells

1Department of Medical-Surgical Sciences and Biotechnologies, Faculty of Pharmacy and Medicine, “Sapienza” University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
2Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Viale Regina Elena 291, 000161 Rome, Italy
3Immunohematology and Transfusion Medicine, San Camillo Forlanini Hospital, Circonvallazione Gianicolense 87, 00152 Rome, Italy
4Department of Medical-Surgical Science and Translational Medicine, Division of Thoracic Surgery, “Sapienza” University of Rome, Sant’Andrea Hospital, Via di Grottarossa 1035, 00189 Rome, Italy
5Department of Experimental Medicine, “Sapienza” University of Rome, Viale Regina Elena 324, 000161 Rome, Italy

Received 19 March 2015; Revised 4 June 2015; Accepted 10 June 2015

Academic Editor: Sebastiano Sciarretta

Copyright © 2015 Camilla Siciliano et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

After fixing with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA Cat. N. 158127). ADMSC cultures were permeabilized with 0.5% Triton X-100 (Sigma) and incubated in blocking buffer (0.2% Gelatin, Sigma) as described for primary antibodies [14]. Negative controls consisted of the secondary antibody alone (all AlexaFluor 488, Invitrogen) added at room temperature for 2 hours. Images were acquired by fluorescent microscope (Leica, software IAS2000). Nuclei were counterstained by DAPI (1:1000, 4’-6’-Diamidino-2-phenylindole, powder ≥98%; Sigma, St. Louis, MO, USA, Cat. N. D9542).

  1. Supplementary Materials