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BioMed Research International
Volume 2015, Article ID 197608, 9 pages
http://dx.doi.org/10.1155/2015/197608
Research Article

Cytotoxicity of Cyclodipeptides from Pseudomonas aeruginosa PAO1 Leads to Apoptosis in Human Cancer Cell Lines

1Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo (UMSNH), Edificio B-3, Ciudad Universitaria, 58030 Morelia, MICH, Mexico
2Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootécnia, UMSNH, 58893 Morelia, MICH, Mexico

Received 14 August 2014; Revised 18 October 2014; Accepted 19 October 2014

Academic Editor: J. Eleazar Barboza-Corona

Copyright © 2015 Dolores Vázquez-Rivera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Effect of cyclodipeptides from Pseudomonas aeruginosa on HeLa and Caco-2 cell viability. HeLa and Caco-2 cells were incubated serum-free medium (SS) containing the CDP mix for 24 h. (A) Viability was determined by the MTT assay and quantitation of fluorescence. Bars represent the mean value ± the standard error (SE) of three independent experiments. One-way analysis of variance was carried out, with Tukey’s post-hoc test; n = 6. Values for SE (P < 0.05) are shown in lower-case letters. (B) Nonlinear regression analysis of dose-response for the inhibition of viability by the CDP mix; 95% confidence interval, P < 0.001. HeLa: 50% inhibitory concentration (IC50) = 0.49 mg/mL; R2 = 0.96. Caco-2: IC50 = 0.75 mg/mL, R2 = 0.93.

  1. Supplementary Material