BioMed Research International

BioMed Research International / 2015 / Article

Research Article | Open Access

Volume 2015 |Article ID 304753 |

Chenghe Wang, Qiangqiang Ge, Zhong Chen, Jia Hu, Fan Li, Xiaodong Song, Hua Xu, Zhangqun Ye, "A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression", BioMed Research International, vol. 2015, Article ID 304753, 13 pages, 2015.

A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression

Academic Editor: Jiing-Kuan Yee
Received03 Jan 2015
Revised09 Mar 2015
Accepted11 Mar 2015
Published30 Mar 2015


We have previously demonstrated that miR-1180-5p has potent ability to upregulate p21 expression by targeting promoter and inhibit bladder cancer. This prompted us to conjecture that a candidate dsRNA (dsP21-397) with perfect complementarity to the miR-1180-5p target site of p21 promoter may also trigger p21 expression. Transfection of dsP21-397 into T24 and EJ cells significantly activated p21 expression at 72 h and the activation presented in a time-course and dose-dependent manner. Moreover, the p21-activated activities of dsP21-397 and miR-1180-5p are not significantly different. Overexpression of p21 downregulated Cyclin D1, CDK4/6, and Cyclin A2 expression, and thereby induced cell cycle arrest and inhibited proliferation. Moreover, dsP21-397 suppressed bladder cancer largely depended on manipulating p21. In conclusion, our study identifies a pair of miRNA-dsRNA mediating endogenous p21 overexpression.

1. Introduction

Bladder cancer (BC) represents one of the most common tumors diagnosed in the United States, with an estimated annual incidence of 74,690 new cases and 15,580 deaths in 2014 [1]. The main characteristic of cancer cells is their increased proliferative activity, which is often caused by aberrations in cell cycle regulation [2]. The (p21) is a broad-acting cyclin-dependent kinase (CDK) inhibitor that binds and inhibits Cyclin-CDK complexes and thereby mediates cell cycle in the G1 phase and leads to proliferation arrest [3, 4]. Therefore, upregulation of p21 is an ideal method of gain-of-function manipulation to suppress cancer cell growth.

RNA interference (RNAi) is an evolutionary conserved gene silencing mechanism in which small RNAs, such as endogenous microRNAs (miRNAs) or exogenous double stranded RNAs (dsRNAs), target homologous mRNA sequences to degrade the mRNAs or inhibit their translation [5, 6]. In contrast, increasing evidences have emerged that miRNAs and dsRNAs can also function to upregulate specific genes expression by targeting promoter, a phenomenon termed as RNA activation (RNAa) [7, 8]. As tumorigenesis may result from functional silence of tumor suppressor genes, enforced expression of these genes by RNAa would otherwise offer great potential for cancer therapy.

Place et al. reported that E-cadherin can be induced through RNAa after transfection of mature miR-373 or the corresponding dsRNA (dsEcad-640) which is fully complementary to promoter sequences [7]. We have also shown that miR-1180-5p holds the capacity to upregulate p21 expression and inhibit BC cells [9]. However, whether a homologous dsRNA perfectly complementary to putative miR-1180-5p target site of p21 promoter can activate p21 expression and suppress BC remains unexplored.

In the present study, we transfect a candidate dsRNA into BC cells and examine the p21 expression. Our results show that the dsRNAs with complete complementarity to the same p21 promoter sequences also significantly inhibit BC cells proliferation and clonogenicity.

2. Materials and Methods

2.1. dsRNAs

All the RNA duplexes which possessed 2-nucleotide 3′ overhangs were chemically synthesized by GenePharma (Shanghai, China). A small interfering RNA (siP21) was used to silence p21 expression and a dsRNA lacking significant homology to all known human sequences (dsControl) was used as a nonspecific control [7, 10]. All the custom dsRNA sequences are listed in Supplementary Table S1 (available online at

2.2. Cell Culture and Transfection

Human BC cell lines T24 and EJ (ATCC) were cultured in RPMI 1640 medium (Hyclone, USA) supplemented with 10% fetal bovine serum (Gibco, USA) in a humidified atmosphere with 5% CO2 at 37°C. The day before transfection, cells were plated to about 60% confluency in medium without antibiotics. dsRNA was transfected at indicated concentrations by using Lipofectamine RNAiMax (Invitrogen, USA) according to the manufacturer’s instructions. Medium was replaced 8 hours later after transfection and then changed daily.

2.3. RNA Isolation and Real-Time PCR

Total cellular RNA from BC cells was extracted by using Trizol reagent (Invitrogen, USA). 500 ng of RNA was used for cDNA synthesis according to the protocol provided by Takara reverse transcription kit (Takara, China). The resulting cDNA was amplified by SYBR Premix Ex Taq II (Takara, China) conducted on the Mx3000P system (Stratagene, USA). The primers included in this study were provided by Invitrogen (Shanghai, China) and listed in Supplementary Table S2. GAPDH was used as internal control to determine the relative expression of target genes’ mRNA. All reactions were performed in triplicates.

2.4. Protein Extraction and Western Blot Analysis

Total proteins were extracted using RIPA lysis buffer supplemented with protease inhibitor Cocktail (Roche, Switzerland). Protein concentrations were calculated by using BCA protein assay kit (Beyotime, China). Equivalent amounts of protein samples (50 μg) of each group were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a PVDF membrane. Membranes were blocked in 5% nonfat dried milk and incubated with the primary antibodies overnight at 4°C. After several washes, membranes were incubated for 1 h at room temperature with corresponding second antibody and detected by enhanced chemiluminescence (ECL) assay kit (Millipore, USA). The primary antibodies were as follows: anti-p21 (1/2000) (Cell Signaling Technology, USA), anti-Cyclin D1 (1/2000) (Affinity, USA), anti-CDK4 (1/1000) (Affinity, USA), anti-CDK6 (1/2000) (Affinity, USA), anti-Cyclin A2 (1/400) (Boster, China), anti-GAPDH (1/500) (Boster, China), and anti-α-tublin (1/500) (Boster, China).

2.5. Clonogenic Survival Assay

After transfection of indicated dsRNA for 24 h, cells were trypsinized, counted, and reseeded in six-well plates at the density of 1000 cells/well for 10 days with complete medium. The medium was changed every 3 days to maintain the cells growth. The colonies were fixed and stained with 0.5% crystal violet (Sigma, USA) for 30 min at room temperature. The colony formation rate was calculated using the following equation: colony formation rate = number of colonies/number of seeded cells × 100%.

2.6. Cell Cycle Analysis

72 h after transfection, cells were harvested by trypsinization and fixed with 70% ethanol at 4°C overnight. Then the cells were washed and incubated with RNase A (0.1 mg/mL) for 30 min at 37°C. Cellular DNA was stained with propidium iodide (PI) (0.05 mg/mL) and analyzed on a FACSort flow cytometer (BD Biosciences, USA). All experiments were performed in triplicate and a total of 10,000 events were analyzed for each sample. The data were processed by CELL Quest software (BD Biosciences, USA).

2.7. Cell Proliferation Assay

Cell proliferation was determined using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, USA) according to the manufacturer’s protocol. Briefly, cells were transfected with dsRNA in a 6-well plate. The following day, the cells were trypsinized and seeded at 1000 cells/well into a new 96-well plate. The plates were then incubated for 5 days and cell growth was measured at daily intervals from the next day to the fifth day. At each time point, 20 μL of CellTiter 96 AQueous One Solution was added to each well and incubated for 2 hour at 37°C. Absorbance was measured on a microplate reader (Bio-Rad, USA) at 490 nm.

2.8. Statistical Analysis

All data were expressed as the mean ± standard deviation (SD) for three independent experiments. Differences between groups were analyzed by -tests using SPSS version 13.0 software (SPSS Inc., Chicago, IL, USA). value < 0.05 was considered to be statistically significant.

3. Results

3.1. dsP21-397 Targets p21 Promoter to Induce Its Expression

We have reported p21 is susceptible to gene induction with miR-1180-5p by targeting its promoter sequences at -397/-379 relative to transcription starting site (Figure 1(a)) [9]. It is speculated that a candidate dsRNA (dsP21-397) fully complementary to the putative miR-1180-5p target site may also have the ability to activate p21 expression (Figure 1(a)). Hereby we transfected synthetic dsP21-397 into T24 and EJ cells and analyzed p21 expression 72 h later. Compared to mock transfection, dsP21-397 caused a 4.03- and 3.35-fold induction in p21 mRNA expression, respectively (Figures 1(b) and 1(c)). This induction was further confirmed by western blot assessment (Figure 1(d)). These results suggest that dsP21-397 holds the capacity to induce p21 expression by targeting promoter.

3.2. Properties of dsP21-397 Mediated p21 Gene Expression

Next, we evaluated the time-course effects of dsP21-397-mediated upregulation of p21 in BC cell lines. As shown in Figures 2(a) and 2(b), induction of p21 mRNA expression in both T24 and EJ cells began to emerge 48 h after transfection and the level continued to increase at 72 h. This time-course feature was further confirmed by immunoblot analysis (Figure 2(c)). The results suggest that enhanced p21 expression may be owing to the direct effect of RNAa. Moreover, to compare the activities of miR-1180-5p and dsP21-397 on activating p21, expression of the gene’s mRNA was quantified by the dose-dependent experiments at 72 h following the two types of small RNAs transfection. Apparently, higher concentrations of miR-1180-5p or dsP21-397 caused more p21 mRNA expression, but the mRNA levels were not significantly different at the same concentrations of the two activators (Figures 2(d)-2(e) and Figure S1).

3.3. dsP21-397 Induces BC Cell Cycle Arrest, Inhibits Proliferation, and Suppresses Colony Formation

To investigate the effect of p21 upregulation induced by dsP21-397 on BC cells development, we analyzed the expression of Cyclin-CDK which manipulates the cell cycle progression. As shown in Figure 3(a), compared with mock and dsControl groups, western blot assay indicated that Cyclin D1, CDK4, CDK6, and Cyclin A2 were significantly decreased at 72 h after dsP21-397 transfection in T24 and EJ cells. The decreases were further verified by densitometric analysis of the immunoblots (Figures 3(b) and 3(c)).

Then we performed flow cytometry to measure cell cycle distribution. In both tested cell lines, transfection with dsP21-397 resulted in a significant increase in the G0/G1 population and a corresponding decrease in the S phase population compared with mock and dsControl treatments (Figure 4(a)). Besides, CellTiter 96 AQueous One Solution Cell Proliferation Assay demonstrated that compared to mock and dsControl groups, both T24 and EJ cells transfected dsP21-397 exhibited progressive retarded growth from day 2 following transfection (Figure 4(b)). However, mock transfected cells maintained similar growth with cells transfected with control dsRNA (Figure 4(b)). We further checked whether transfection of dsP21-397 exerts an inhibitory influence on cell proliferation ability in BC cells. Apparently, colony formation assay revealed that dsP21-397 transfected cells formed colonies significantly fewer in number and smaller in size (Figure 4(c)). Moreover, the colony rates of dsP21-397 transfected cells were remarkably lower than mock and dsControl treatments (Figure 4(d)). These results are in agreement with previous studies in which activation of p21 leads to G0/G1 arrest and decline in cell proliferation [11, 12].

3.4. dsP21-397 Decreases Cyclin D1-CDK4/6 and Cyclin A2 Expression by Activating p21

To validate that dsP21-397 suppresses Cyclin-CDK expression mainly by upregulating p21, we silenced p21 expression by transfecting small interfering RNA siP21 in T24 and EJ cells [13]. The results showed that cotransfection of dsP21-397 and siP21 in BC cells markedly abrogated the activating effect mediated by dsP21-397 at both mRNA and protein levels at 72 h (Figures 5(a) and 5(b)). Then, we examined the expression of p21 downstream effectors in BC cells. As seen from Figure 5(c), dsP21-397 failed to downregulate Cyclin D1, CDk4/6, and Cyclin A2 expression after siP21 cotreatments. Densitometric analysis quantification of immunoblots further proved that (Figures 5(d) and 5(e)). This suggests that dsP21-397 inhibits Cyclin D1, CDK4/6, and Cyclin A2 expression largely depending on modulating p21.

3.5. dsP21-397 Inhibits BC Cells Mainly by Upregulation of p21

Next, we verify whether depletion of p21 could affect the inhibitory function of dsP21-397 in T24 and EJ cells. Remarkably, p21 silencing significantly attenuated G0/G1 cycle arrest mediated by dsP21-397 in both cell lines (Figure 6(a)). Moreover, the antiproliferative effect resulting from Cyclin D1, CDK4/6, and Cyclin A2 downregulation was evidently alleviated by p21 silencing (Figure 6(b)). In addition, the colony formation ability of the BC cells was also restored (Figures 6(c) and 6(d)). Taken together, these experiments strongly implied that p21 is mainly responsible for BC cells suppression by dsP21-397.

4. Discussion

In the present study, we identify that a synthetic dsRNA (dsP21-397), which is completely complementary to putative miR-1180-5p target site of p21 promoter, exhibits considerable potency to activate p21 expression in BC cells. And this activation presents a time-course and dose-dependent effect. Moreover, dsP21-397 possesses a similar capacity with miR-1180-5p to enhance p21 expression. Several Cyclin-CDK genes (Cyclin D1, CDK4/6, and Cyclin A2) associated with cell cycle are downregulated following dsP21-397 transfection. Hereby, transfection of dsP21-397 causes BC cells’ cycle arrest and impedes proliferation. In addition, this dsRNA’s antitumor function is mainly achieved by regulating p21 expression.

One distinctive feature of cancer cells is their sustained proliferative activity; thus blockade of cell cycle is regarded as effective strategy for cancer therapy [14]. Cell cycle is controlled by Cyclin-CDK. Inhibition of Cyclin D1-CDK4/6 complexes activity elicits reduction in G0/G1-to-S phase progression [15]. Furthermore, Cyclin A2 plays a decisive role in regulation of S phase and its deficiency will make cells accumulation in G0/G1 and decreases entry into S phase [16]. It is now well known that p21 is a powerful CDK inhibitor involved in many antigrowth pathways including promoting cell cycle arrest [17]. The p21 protein has a cyclin binding motif and inhibits Cyclin-CDK complexes, such as Cyclin D1-CDK4/6 and Cyclin A2-CDK2, by direct interaction [18]. By the way, we have shown that several miRNAs mediated p21 overexpression potently accelerates BC cells apoptosis [9]. However, saRNAs also own the proapoptotic effect on BC cells due to the similar approach [1921].

Earlier studies indicated that p21 is frequently low expressed in BC cells and correlates with prognosis [22, 23]; enhancement of its expression by RNAa would undoubtedly exert potent antitumor capacity [9, 19, 24]. Moreover, we and others have reported that p21 expression could be significantly induced by multiple dsRNA or miRNAs in variable levels by targeting different regions of promoter [9, 25].

RNAi is a posttranscriptional gene silencing mechanism while RNAa functions at the transcriptional level [8, 26]. RNAi and RNAa regulate target genes either negatively or positively, presenting opposite effects. Unlike RNAi, the exact mechanism of RNAa is not well-characterized. However, to our knowledge, this RNAa phenomenon required both transcriptional and epigenetic alterations. The dsRNA is first loaded onto a cytoplasmic Argonaute. Then the sense strand is removed and an active Argonaute-RNA complex formed. The complex is further proceeded to bind complementary promoter DNA guided by the antisense strand. Afterwards, histone modifying enzymes are enriched at the promoter and activate transcription via epigenetic modifications [27, 28].

As we know, high complementarity of sequences between miRNA/dsRNA and its target promoter is essential for RNAa [29]. Previous studies have reported that endogenous miRNAs partially complementary to promoters still possess the ability to activate genes but excess mismatch is not tolerable [7, 9, 30]. So it can be concluded that a synthetic dsP21-397 fully complementary to p21 promoter would induce p21 expression more easily. Surprisingly, even with a higher complementarity to p21 promoter, dsP21-397 did not induce more p21 expression than miR-1180-5p at the same transfecting concentration in our study. The exact mechanism is unclear and future research is needed.

Recently, noncoding RNAs, such as enhancer RNAs (eRNAs) and natural antisense transcripts (NATs), have been recognized to regulate genes expression by interacting with promoter. However, their gene modulating modes are distinct from RNAa. Generally, eRNAs are transcribed from enhancers and contribute to enhancers function by mediating enhancer-promoter interaction [31, 32]. Sequences of NATs are complementary to that of sense strand which is defined as mRNA and encode proteins [33]. Besides, NATs can be found in promoters, introns, exons, and untranslated regions of the genome [34]. Functionally, NATs can suppress transcription by chromatin remodeling, transcriptional interference, RNA masking, and so on [34].

BC arising contains numerous genetic alternations, such as inactivation of tumor suppressor genes [35, 36]. Therefore, reactivation of specific tumor suppressor p21 will surely offer a promising method for cancer therapeutics. Additionally, Li et al. have demonstrated that RNAa is safe for manipulating certain gene expression without altering expression profile of other nonspecific genes [8].

5. Conclusions

Taken together, our results provide evidences that dsP21-397 stimulated p21 expression by targeting promoter-derived sequences which is identical to miR-1180-5p target site. Moreover, dsP21-397 also has the capacity to induce cell cycle arrest and inhibits proliferation of BC cells. Our study first identifies a pair of miRNA-dsRNA which both mediate endogenous p21 overexpression and suppress BC.


The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the paper.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.


This work was supported by the National Natural Science Foundation of China (Grant no. 81372759), China.

Supplementary Materials

Supplementary Table S1: All dsRNAs used in present study werechemically synthesized with dTdT 3'overhangs. The dsP21-397 complementary to the p21 promoter at sequences position -397 relative to the transcription starting site was used to activate p21 expression. The control dsRNA (dsControl) was designed to lack significant homology to all known human genes' sequences. The dsRNA (siP21) was used to silence p21 expression by targeting p21 mRNA 3' untranslated regions.

Supplementary Table S2: The primes were used to detect p21 and GAPDH mRNA relative expression levels in BC cell lines by real-time PCR.

Figure S1: In order to compare the activating activities between miR-1180-5p and dsP21-397, we transfected T24 and EJ cells with miR-1180-5p or dsP21-397 at 0nM, 25nM, 50nM and 100nM, respectively. 72 h later, p21 mRNA was determined by real-time PCR and the miR-1180-5p induced p21 mRNA expression levels were normalized to dsP21-397 groups at the same concentrations. As shown in Figure S1A and S1B, the p21 expression was not significantly different between miR-1180-5p and dsP21-397 at the same transfecting concentrations in T24 and EJ cells.

  1. Supplementary Material


  1. R. Siegel, J. Ma, Z. Zou, and A. Jemal, “Cancer statistics, 2014,” CA: A Cancer Journal for Clinicians, vol. 64, no. 1, pp. 9–29, 2014. View at: Publisher Site | Google Scholar
  2. S. J. Yun, S.-K. Moon, and W.-J. Kim, “Investigational cell cycle inhibitors in clinical trials for bladder cancer,” Expert Opinion on Investigational Drugs, vol. 22, no. 3, pp. 369–377, 2013. View at: Publisher Site | Google Scholar
  3. N. G. Starostina and E. T. Kipreos, “Multiple degradation pathways regulate versatile CIP/KIP CDK inhibitors,” Trends in Cell Biology, vol. 22, no. 1, pp. 33–41, 2012. View at: Publisher Site | Google Scholar
  4. L. A. Stivala, O. Cazzalini, and E. Prosperi, “The cyclin-dependent kinase inhibitor p21CDKN1A as a target of anti-cancer drugs,” Current Cancer Drug Targets, vol. 12, no. 2, pp. 85–96, 2012. View at: Publisher Site | Google Scholar
  5. Z. Wang, D. D. Rao, N. Senzer, and J. Nemunaitis, “RNA interference and cancer therapy,” Pharmaceutical Research, vol. 28, no. 12, pp. 2983–2995, 2011. View at: Publisher Site | Google Scholar
  6. M. Ghildiyal and P. D. Zamore, “Small silencing RNAs: an expanding universe,” Nature Reviews Genetics, vol. 10, no. 2, pp. 94–108, 2009. View at: Publisher Site | Google Scholar
  7. R. F. Place, L. C. Li, D. Pookot, E. J. Noonan, and R. Dahiya, “MicroRNA-373 induces expression of genes with complementary promoter sequences,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 5, pp. 1608–1613, 2008. View at: Publisher Site | Google Scholar
  8. L.-C. Li, S. T. Okino, H. Zhao et al., “Small dsRNAs induce transcriptional activation in human cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 46, pp. 17337–17342, 2006. View at: Publisher Site | Google Scholar
  9. C. Wang, Z. Chen, Q. Ge et al., “Up-regulation of p21 by miRNAs and its implications in bladder cancer cells,” FEBS Letters, vol. 588, no. 24, pp. 4654–4664, 2014. View at: Publisher Site | Google Scholar
  10. M. Miyazaki, M. Sakaguchi, I. Akiyama, Y. Sakaguchi, S. Nagamori, and N.-H. Huh, “Involvement of interferon regulatory factor 1 and S100C/A11 in growth inhibition by transforming growth factor beta 1 in human hepatocellular carcinoma cells,” Cancer Research, vol. 64, no. 12, pp. 4155–4161, 2004. View at: Publisher Site | Google Scholar
  11. H. Y. Park, G.-Y. Kim, S.-K. Moon, W. J. Kim, Y. H. Yoo, and Y. H. Choi, “Fucoidan inhibits the proliferation of human urinary bladder cancer T24 cells by blocking cell cycle progression and inducing apoptosis,” Molecules, vol. 19, no. 5, pp. 5981–5998, 2014. View at: Publisher Site | Google Scholar
  12. R. W. Zhang, Y. L. Wang, J. X. Li, H. L. Jin, S. J. Song, and C. S. Huang, “The chinese herb isolate yuanhuacine (YHL-14) induces G2/M arrest in human cancer cells by up-regulating p21 protein expression through an p53 protein-independent cascade,” The Journal of Biological Chemistry, vol. 289, no. 10, pp. 6394–6403, 2014. View at: Publisher Site | Google Scholar
  13. Q. Li, J. Li, T. Wen et al., “Overexpression of HMGB1 in melanoma predicts patient survival and suppression of HMGB1 induces cell cycle arrest and senescence in association with p21 (Waf1/Cip1) up-regulation via a p53-independent, Sp1-dependent pathway,” Oncotarget, vol. 5, no. 15, pp. 6387–6403, 2014. View at: Google Scholar
  14. T. Sperka, J. Wang, and K. L. Rudolph, “DNA damage checkpoints in stem cells, ageing and cancer,” Nature Reviews Molecular Cell Biology, vol. 13, no. 9, pp. 579–590, 2012. View at: Publisher Site | Google Scholar
  15. A. J. Obaya, I. Kotenko, M. D. Cole, and J. M. Sedivy, “The proto-oncogene c-myc acts through the cyclin-dependent kinase (Cdk) inhibitor p27Kip1to facilitate the activation of Cdk4/6 and early G1 phase progression,” The Journal of Biological Chemistry, vol. 277, no. 34, pp. 31263–31269, 2002. View at: Publisher Site | Google Scholar
  16. L. Dai, Y. Liu, J. Liu et al., “A novel CyclinE/CyclinA-CDK inhibitor targets p27Kip1 degradation, cell cycle progression and cell survival: implications in cancer therapy,” Cancer Letters, vol. 333, no. 1, pp. 103–112, 2013. View at: Publisher Site | Google Scholar
  17. T. Abbas and A. Dutta, “P21 in cancer: intricate networks and multiple activities,” Nature Reviews Cancer, vol. 9, no. 6, pp. 400–414, 2009. View at: Publisher Site | Google Scholar
  18. J. Chen, P. Saha, S. Kornbluth, B. D. Dynlacht, and A. Dutta, “Cyclin-binding motifs are essential for the function of p21CIP1,” Molecular and Cellular Biology, vol. 16, no. 9, pp. 4673–4682, 1996. View at: Google Scholar
  19. Z. Chen, R. F. Place, Z.-J. Jia, D. Pookot, R. Dahiya, and L.-C. Li, “Antitumor effect of dsRNA-induced p21WAF1/CIP1 gene activation in human bladder cancer cells,” Molecular Cancer Therapeutics, vol. 7, no. 3, pp. 698–703, 2008. View at: Publisher Site | Google Scholar
  20. K. Yang, X. Y. Zheng, J. Qin et al., “Up-regulation of p21WAF1/Cip1 by saRNA induces G1-phase arrest and apoptosis in T24 human bladder cancer cells,” Cancer Letters, vol. 265, no. 2, pp. 206–214, 2008. View at: Publisher Site | Google Scholar
  21. H. Wang, W. Liu, J. Jin, L. Zhou, L. Liang, and Y. Guo, “M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway,” Journal of Cancer Research and Therapeutics, vol. 9, no. 1, pp. 54–59, 2013. View at: Publisher Site | Google Scholar
  22. J. P. Stein, D. A. Ginsberg, G. D. Grossfeld et al., “Effect of p21(WAF1/CIP1) expression on tumor progression in bladder cancer,” Journal of the National Cancer Institute, vol. 90, no. 14, pp. 1072–1079, 1998. View at: Publisher Site | Google Scholar
  23. H. M. Behnsawy, H. Miyake, M. A. Abdalla, M. A. Sayed, A. E.-F. I. Ahmed, and M. Fujisawa, “Expression of cell cycle-associated proteins in non-muscle-invasive bladder cancer: correlation with intravesical recurrence following transurethral resection,” Urologic Oncology: Seminars and Original Investigations, vol. 29, no. 5, pp. 495–501, 2011. View at: Publisher Site | Google Scholar
  24. M. Kang, G. Yang, R. F. Place et al., “Intravesical delivery of small activating RNA formulated into lipid nanoparticles inhibits orthotopic bladder tumor growth,” Cancer Research, vol. 72, no. 19, pp. 5069–5079, 2012. View at: Publisher Site | Google Scholar
  25. R. F. Place, J. Wang, E. J. Noonan et al., “Formulation of small activating RNA into lipidoid nanoparticles inhibits xenograft prostate tumor growth by inducing p21 expression,” Molecular Therapy—Nucleic Acids, vol. 1, article e15, 2012. View at: Publisher Site | Google Scholar
  26. S. M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl, “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells,” Nature, vol. 411, no. 6836, pp. 494–498, 2001. View at: Publisher Site | Google Scholar
  27. L. Zheng, L. Wang, J. Gan, and H. Zhang, “RNA activation: promise as a new weapon against cancer,” Cancer Letters, vol. 355, no. 1, pp. 18–24, 2014. View at: Publisher Site | Google Scholar
  28. V. Huang, J. Zheng, Z. Qi et al., “Ago1 Interacts with RNA polymerase II and binds to the promoters of actively transcribed genes in human cancer cells,” PLoS Genetics, vol. 9, no. 9, Article ID e1003821, 2013. View at: Google Scholar
  29. V. Portnoy, V. Huang, R. F. Place, and L.-C. Li, “Small RNA and transcriptional upregulation,” Wiley Interdisciplinary Reviews: RNA, vol. 2, no. 5, pp. 748–760, 2011. View at: Publisher Site | Google Scholar
  30. V. Huang, R. F. Place, V. Portnoy et al., “Upregulation of Cyclin B1 by miRNA and its implications in cancer,” Nucleic Acids Research, vol. 40, no. 4, pp. 1695–1707, 2012. View at: Publisher Site | Google Scholar
  31. M. T. Y. Lam, W. Li, M. G. Rosenfeld, and C. K. Glass, “Enhancer RNAs and regulated transcriptional programs,” Trends in Biochemical Sciences, vol. 39, no. 4, pp. 170–182, 2014. View at: Publisher Site | Google Scholar
  32. N. Hah, S. Murakami, A. Nagari, C. G. Danko, and W. Lee Kraus, “Enhancer transcripts mark active estrogen receptor binding sites,” Genome Research, vol. 23, no. 8, pp. 1210–1223, 2013. View at: Publisher Site | Google Scholar
  33. M. Nishizawa, T. Okumura, Y. Ikeya, and T. Kimura, “Regulation of inducible gene expression by natural antisense transcripts,” Frontiers in Bioscience, vol. 17, no. 3, pp. 938–958, 2012. View at: Publisher Site | Google Scholar
  34. K. Li and R. Ramchandran, “Natural antisense transcript: a concomitant engagement with protein-coding transcript,” Oncotarget, vol. 1, no. 6, pp. 447–452, 2010. View at: Google Scholar
  35. The Cancer Genome Atlas Research Network, “Comprehensive molecular characterization of urothelial bladder carcinoma,” Nature, vol. 507, no. 7492, pp. 315–322, 2014. View at: Publisher Site | Google Scholar
  36. X.-R. Wu, “Urothelial tumorigenesis: a tale of divergent pathways,” Nature Reviews Cancer, vol. 5, no. 9, pp. 713–725, 2005. View at: Publisher Site | Google Scholar

Copyright © 2015 Chenghe Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

More related articles

 PDF Download Citation Citation
 Download other formatsMore
 Order printed copiesOrder

Related articles

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.