BioMed Research International / 2015 / Article / Fig 4

Research Article

A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression

Figure 4

dsP21-397 induces BC cells cycle arrest and inhibits proliferation. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs for 72 h. mock sample was transfected in the absence of dsRNA. (a) Cell cycle distribution was conducted using flow cytometry with PI stained. (b) Viable cells were measured from day 1 to 5 following transfection using the CellTiter 96 AQueous One Solution Cell Proliferation Assay kit. Results were plotted as OD values. , , and compared to mock and dsControl groups at the same time point. (c) Representative photographs of colony formation assay. (d) Quantification of the cell colonies formation. and compared to mock and dsControl groups.

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