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BioMed Research International
Volume 2015 (2015), Article ID 403413, 16 pages
Research Article

Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model

1Medical Life System, Faculty of Life and Medical Science, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0394, Japan
2Graduate School of Medicine for the Master’s Course, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan
3Kusatsu General Hospital, 1660 Yabase-cho, Kusatsu, Shiga 525-8585, Japan
4Department of Economics, Doshisha University, Imadegawa-Karasuma, Kamigyo-ku, Kyoto 602-8580, Japan
5Biomedical Material Research Center, Doshisha University, 1-3 Tatara Miyakodani, Kyotanabe, Kyoto 610-0321, Japan

Received 24 July 2014; Accepted 17 September 2014

Academic Editor: Janet H. Fitton

Copyright © 2015 Mari Matoba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Postoperative intra-abdominal or intrathoracic adhesions sometimes cause significant morbidity. We have designed three types of alginate-based treatments using strongly cross-linked (SL), weakly cross-linked (WL), and non-cross-linked (NL) alginate with calcium gluconate. In rat experiments, we compared the antiadhesive effects of the three types of alginate-based treatments, fibrin glue treatment (a standard treatment), and no treatment against adhesions caused by polyglycolic acid (PGA) mesh (PGA-induced adhesions). The antiadhesive materials were set on the PGA sheet fixed on the parietal peritoneum of the abdomen. Fifty-six days later, the adhesions were evaluated macroscopically by the adhesion scores and microscopically by hematoxylin-eosin staining and immunostaining. We also tested the fibroblast growth on the surface of the antiadhesive materials in vitro. The antiadhesive effects of WL and NL were superior to the no treatment and fibrin glue treatment. A microscopic evaluation confirmed that the PGA sheet was covered by a peritoneal layer constructed of well-differentiated mesothelial cells, and the inflammation was most improved in the NL and WL. The fibroblast growth was inhibited most on the surfaces of the NL and WL. These results suggest that either the WL or NL treatments are suitable for preventing PGA-induced adhesions compared to SL or the conventional treatment.