(a)
(b)
(c)
(d)
Figure 2: TCEE induced cell cycle arrest and activation of caspase-3. (a) The cell cycle distribution was analyzed by FACS using PI staining on Hep3B and HepJ5 cells which were treated with 1 or 2 mg/mL TCEE for 24 hr. (b) Bar charts for the comparison of control or TCEE treated cells. Data were presented as mean plus standard deviation. indicated statistical significance compared with the control group ( by Student’s -test). (c) Expressions of P21 and P27 and cleaved caspase-3 on Hep3B and HepJ5 cells with 0.5 or 1.0 mg/mL TCEE treatment for 48 hr were determined by western blotting analysis. GAPDH was used as the normalization control. The semiquantitative data were shown in Table 2. (d) Cell apoptosis was analyzed by the FACS analysis using the double-staining of PI and FITC-labelled annexin V on Hep3B and HepJ5 cells which were treated with 1 or 2 mg/mL TCEE for 24 hr.