The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type

Figure 3

Identification of 6q21 and PRDM1 gene deletion by dual-color FISH and PRDM1 protein expression by Western blot in cell lines. (a) Dual-color FISH analysis was performed in NK/T cell lymphoma cell lines (YT, NK92, and NKL) and control cell line (K562, human chronic myelogenous leukemia cell) as described in Figure 1. YT cells with strong PRDM1 protein expression showed no deletion of 6q21 and PRDM1 gene, while NK92 and NKL cells with weak PRDM1 expression displayed the heterozygous codeletion of 6q21 and PRDM1. In addition, control cell line K562 lack of PRDM1 expression showed no deletion of 6q21 or PRDM1. All are shown at 1000x magnification. (b) Representative picture of PRDM1 protein expression by Western blot in YT, NK92, NKL, and K562 cells. ^#No deletion of 6q21 or PRDM1 gene. ^△The heterozygous codeletion of 6q21 and PRDM1 gene.

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