Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 513875, 18 pages
http://dx.doi.org/10.1155/2015/513875
Research Article

HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa

1Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India
2Human Genetics Laboratory, Department of Bioscience, Jamia Millia Islamia, New Delhi 110025, India

Received 22 May 2015; Revised 6 August 2015; Accepted 19 August 2015

Academic Editor: Kanjoormana A. Manu

Copyright © 2015 Md. Nasar Mallick et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. L. Siegel, K. D. Miller, and A. Jemal, “Cancer statistics,” CA: A Cancer Journal for Clinicians, vol. 65, no. 1, pp. 5–29, 2015. View at Publisher · View at Google Scholar
  2. Z. Wang, “Roles of K+ channels in regulating tumour cell proliferation and apoptosis,” Pflugers Archiv: European Journal of Physiology, vol. 448, no. 3, pp. 274–286, 2004. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Gerl and D. L. Vaux, “Apoptosis in the development and treatment of cancer,” Carcinogenesis, vol. 26, no. 2, pp. 263–270, 2005. View at Publisher · View at Google Scholar · View at Scopus
  4. T. M. Beer and J. S. Bubalo, “Complications of chemotherapy for prostate cancer,” Seminars in Urologic Oncology, vol. 19, no. 3, pp. 222–230, 2001. View at Google Scholar · View at Scopus
  5. R. C. F. Leonard, S. Williams, A. Tulpule, A. M. Levine, and S. Oliveros, “Improving the therapeutic index of anthracycline chemotherapy: focus on liposomal doxorubicin (Myocet),” Breast, vol. 18, no. 4, pp. 218–224, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. C. W. Benjamin, R. R. Hiebsch, and D. A. Jones, “Caspase activation in MCF7 cells responding to etoposide treatment,” Molecular Pharmacology, vol. 53, no. 3, pp. 446–450, 1998. View at Google Scholar · View at Scopus
  7. J. Zargan, M. Sajad, S. Umar, M. Naime, S. Ali, and H. A. Khan, “Scorpion (Androctonus crassicauda) venom limits growth of transformed cells (SH-SY5Y and MCF-7) by cytotoxicity and cell cycle arrest,” Experimental and Molecular Pathology, vol. 91, no. 1, pp. 447–454, 2011. View at Publisher · View at Google Scholar · View at Scopus
  8. B. Patwardhan and M. Gautam, “Botanical immunodrugs: scope and opportunities,” Drug Discovery Today, vol. 10, no. 7, pp. 495–502, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. T. Sidiq, A. Khajuria, P. Suden et al., “Possible role of macrophages induced by an irridoid glycoside (RLJ-NE-299A) in host defense mechanism,” International Immunopharmacology, vol. 11, no. 1, pp. 128–135, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. O. Kayser, K. N. Masihi, and A. F. Kiderlen, “Natural products and synthetic compounds as immunomodulators,” Expert Review of Anti-Infective Therapy, vol. 1, no. 2, pp. 319–335, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. P. Anand, A. B. Kunnumakkara, K. B. Harikumar, K. S. Ahn, V. Badmaev, and B. B. Aggarwal, “Modification of cysteine residue in p65 subunit of nuclear factor-κB (NF-κB) by picroliv suppresses NF-κB-regulated gene products and potentiates apoptosis,” Cancer Research, vol. 68, no. 21, pp. 8861–8870, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. V. Rajkumar, G. Guha, and R. A. Kumar, “Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts,” Food and Chemical Toxicology, vol. 49, no. 2, pp. 363–369, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. A. Russo, A. A. Izzo, V. Cardile, F. Borrelli, and A. Vanella, “Indian medicinal plants as antiradicals and DNA cleavage protectors,” Phytomedicine, vol. 8, no. 2, pp. 125–132, 2001. View at Publisher · View at Google Scholar · View at Scopus
  14. A. Ray, S. R. Chaudhuri, B. Majumdar, and S. K. Bandyopadhyay, “Antioxidant activity of ethanol extract of rhizome of Picrorhiza kurroa on indomethacin induced gastric ulcer during healing,” Indian Journal of Clinical Biochemistry, vol. 17, no. 2, pp. 44–51, 2002. View at Publisher · View at Google Scholar · View at Scopus
  15. A. A. Patil, B. S. Sachin, D. B. Shinde, and P. S. Wakte, “Supercritical CO2 assisted extraction and LC-MS identification of picroside i and picroside II from Picrorhiza kurroa,” Phytochemical Analysis, vol. 24, no. 2, pp. 97–104, 2013. View at Publisher · View at Google Scholar · View at Scopus
  16. S. S. Tiwari, M. M. Pandey, S. Srivastava, and A. Rawat, “TLC densitometric quantification of picrosides (picroside-I and picroside-II) in Picrorhiza kurroa and its substitute Picrorhiza scrophulariiflora and their antioxidant studies,” Biomedical Chromatography, vol. 26, no. 1, pp. 61–68, 2012. View at Publisher · View at Google Scholar · View at Scopus
  17. T. Kawoosa, H. Singh, A. Kumar et al., “Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa,” Functional & Integrative Genomics, vol. 10, no. 3, pp. 393–404, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. H. Stuppner and H. Wagner, “New cucurbitacin glycosides fom Picrorhiza kurrooa,” Planta Medica, vol. 55, no. 6, pp. 559–563, 1989. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Sturm and H. Stuppner, “Analysis of cucurbitacins in medicinal plants by high-pressure liquid chromatography-mass spectrometry,” Phytochemical Analysis, vol. 11, no. 2, pp. 121–127, 2000. View at Publisher · View at Google Scholar · View at Scopus
  20. N. Sindhu, T. Pratima, and W. Ashwini, “Isolation of a cucurbitacin from Picrorhiza Kurroa by column chromatography and its characterization,” Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 2, no. 3, pp. 149–156, 2011. View at Google Scholar · View at Scopus
  21. F. Engels, B. F. Renirie, B. A. Hart, R. P. Labadie, and F. P. Nijkamp, “Effects of apocynin, a drug isolated from the roots of Picrorhiza kurroa, on arachidonic acid metabolism,” FEBS Letters, vol. 305, no. 3, pp. 254–256, 1992. View at Publisher · View at Google Scholar · View at Scopus
  22. Y. Kong, J. Chen, Z. Zhou, H. Xia, M.-H. Qiu, and C. Chen, “Cucurbitacin e induces cell cycle G2/M phase arrest and apoptosis in triple negative breast cancer,” PLoS ONE, vol. 9, no. 7, Article ID e103760, 2014. View at Publisher · View at Google Scholar · View at Scopus
  23. A. A. Alghasham, “Cucurbitacins—a promising target for cancer therapy,” International Journal of Health Sciences, vol. 7, no. 1, pp. 77–89, 2013. View at Publisher · View at Google Scholar
  24. A. Aribi, S. Gery, D. H. Lee et al., “The triterpenoid cucurbitacin B augments the antiproliferative activity of chemotherapy in human breast cancer,” International Journal of Cancer, vol. 132, no. 12, pp. 2730–2737, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. S. Duangmano, P. Sae-Lim, A. Suksamrarn, F. E. Domann, and P. Patmasiriwat, “Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation,” BMC Complementary Alternative Medicine, vol. 12, article 185, 12 pages, 2012. View at Publisher · View at Google Scholar
  26. J.-S. Zhu, D.-Y. Ouyang, Z.-J. Shi, L.-H. Xu, Y.-T. Zhang, and X.-H. He, “Cucurbitacin B induces cell cycle arrest, apoptosis and autophagy associated with G actin reduction and persistent activation of cofilin in Jurkat cells,” Pharmacology, vol. 89, no. 5-6, pp. 348–356, 2012. View at Publisher · View at Google Scholar · View at Scopus
  27. J.-J. Li, Y.-T. Zhang, D.-Y. Ouyang, L.-H. Xu, H.-B. Mo, and X.-H. He, “Suppression of STAT3 phosphorylation enhances the cytotoxicity of cucurbitacin B in B16F10 melanoma cells,” African Journal of Pharmacy and Pharmacology, vol. 6, no. 21, pp. 1545–1554, 2012. View at Publisher · View at Google Scholar
  28. C. Boykin, G. Zhang, Y.-H. Chen et al., “Cucurbitacin IIa: a novel class of anti-cancer drug inducing non-reversible actin aggregation and inhibiting survivin independent of JAK2/STAT3 phosphorylation,” British Journal of Cancer, vol. 104, no. 5, pp. 781–789, 2011. View at Publisher · View at Google Scholar · View at Scopus
  29. K. T. Chan, F. Y. Meng, Q. Li et al., “Cucurbitacin B induces apoptosis and S phase cell cycle arrest in BEL-7402 human hepatocellular carcinoma cells and is effective via oral administration,” Cancer Letters, vol. 294, no. 1, pp. 118–124, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. A. C. de Almeida, O. C. Marques, C. Arslanian, A. Condino-Neto, and V. F. Ximenes, “4-Fluoro-2-methoxyphenol, an apocynin analog with enhanced inhibitory effect on leukocyte oxidant production and phagocytosis,” European Journal of Pharmacology, vol. 660, no. 2-3, pp. 445–453, 2011. View at Publisher · View at Google Scholar · View at Scopus
  31. C. J. Henrich, C. L. Thomas, A. D. Brooks et al., “Effects of cucurbitacins on cell morphology are associated with sensitization of renal carcinoma cells to TRAIL-induced apoptosis,” Apoptosis, vol. 17, no. 1, pp. 79–89, 2012. View at Publisher · View at Google Scholar · View at Scopus
  32. S. Yasuda, S. Yogosawa, Y. Izutani, Y. Nakamura, H. Watanabe, and T. Sakai, “Cucurbitacin B induces G2 arrest and apoptosis via a reactive oxygen species-dependent mechanism in human colon adenocarcinoma SW480 cells,” Molecular Nutrition & Food Research, vol. 54, no. 4, pp. 559–565, 2010. View at Publisher · View at Google Scholar · View at Scopus
  33. Y. Dong, B. Lu, X. Zhang et al., “Cucurbitacin E, a tetracyclic triterpenes compound from Chinese medicine, inhibits tumor angiogenesis through VEGFR2-mediated Jak2-STAT3 signaling pathway,” Carcinogenesis, vol. 31, no. 12, pp. 2097–2104, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. D. Rathee, M. Thanki, S. Bhuva, S. Anandjiwala, and R. Agrawal, “Iridoid glycosides-Kutkin, Picroside I, and Kutkoside from Picrorrhiza kurroa Benth inhibits the invasion and migration of MCF-7 breast cancer cells through the down regulation of matrix metalloproteinases,” Arabian Journal of Chemistry, vol. 6, no. 1, pp. 49–58, 2013. View at Publisher · View at Google Scholar · View at Scopus
  35. R. Parveen, F. J. Ahmad, Z. Iqbal, M. Singh, Y. T. Kamal, and S. Ahmad, “Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC,” Acta Chromatographica, vol. 26, no. 2, pp. 391–400, 2014. View at Publisher · View at Google Scholar · View at Scopus
  36. Y. T. Kamal, S. M. Musthaba, M. Singh et al., “Development and validation of HPLC method for simultaneous estimation of piperine and guggulsterones in compound Unani formulation (tablets) and a nanoreservoir system,” Biomedical Chromatography, vol. 26, no. 10, pp. 1183–1190, 2012. View at Publisher · View at Google Scholar · View at Scopus
  37. Pharmacopoeia of India, Ministry of Health, Government of India, 2nd edition, 1966.
  38. R. Asirvatham and A. J. M. Christina, “Anticancer activity of Drosera indica L., on Dalton's Lymphoma Ascites (DLA) bearing mice,” Journal of Intercultural Ethnopharmacology, vol. 2, no. 1, pp. 9–14, 2013. View at Google Scholar
  39. R. Parveen, F. J. Ahmad, Z. Iqbal, M. Samim, and S. Ahmad, “Solid lipid nanoparticles of anticancer drug andrographolide: formulation, in vitro and in vivo studies,” Drug Development and Industrial Pharmacy, vol. 40, no. 9, pp. 1206–1212, 2014. View at Publisher · View at Google Scholar · View at Scopus
  40. M. I. Sriram, S. B. M. Kanth, K. Kalishwaralal, and S. Gurunathan, “Antitumor activity of silver nanoparticles in Dalton's lymphoma ascites tumor model,” International Journal of Nanomedicine, vol. 5, no. 1, pp. 753–762, 2010. View at Publisher · View at Google Scholar · View at Scopus
  41. V. Zuco, R. Supino, S. C. Righetti et al., “Selective cytotoxicity of betulinic acid on tumor cell lines, but not on normal cells,” Cancer Letters, vol. 175, no. 1, pp. 17–25, 2002. View at Publisher · View at Google Scholar · View at Scopus
  42. Y. T. Ho, C. C. Lu, J. S. Yang et al., “Berberine induced apoptosis via promoting the expression of caspase-8, 9 and 3, apoptosis-inducing factor and endonuclease G in SCC-4 human tongue squamous carcinoma cancer cells,” Anticancer Research, vol. 29, no. 10, pp. 4063–4070, 2009. View at Google Scholar
  43. N. Armania, L. S. Yazan, S. N. Musa et al., “Dillenia suffruticosa exhibited antioxidant and cytotoxic activity through induction of apoptosis and G2/M cell cycle arrest,” Journal of Ethnopharmacology, vol. 146, no. 2, pp. 525–535, 2013. View at Publisher · View at Google Scholar · View at Scopus