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BioMed Research International
Volume 2015, Article ID 594751, 12 pages
Research Article

Primary Murine Myotubes as a Model for Investigating Muscular Dystrophy

1Department of Women’s and Children’s Health, Karolinska University Hospital, Solna, 17176 Stockholm, Sweden
2Center for Molecular Medicine, Karolinska University Hospital, Solna, 17176 Stockholm, Sweden
3Federal Almazov Medical Research Centre, 2 Akkuratova Street, Saint Petersburg 197341, Russia
4Institute of translational Medicine, ITMO University, 14 Birzjevaya Line, Saint Petersburg 199034, Russia
5Department of Physiology and Pharmacology, Karolinska Institutet, 17177 Stockholm, Sweden

Received 31 December 2014; Accepted 11 March 2015

Academic Editor: Sachchida Nand Pandey

Copyright © 2015 Natalia Smolina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

This movie depicts spontaneously contracting myotubes. Satellite cells were isolated from m. soleus and induced to differentiation for 4 days. Obtained myotubes were amenable to contraction without extra stimuli. Multiple foci of contraction can be observed.

  1. Supplementary Movie