BioMed Research International / 2015 / Article / Tab 5

Research Article

A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

Table 5

Comparison of Se and Sp for flavivirus MIA, ELISA, and VNT on horse field sera.

Virus identified MIA/ELISAMIA/VNTNumber of field sera tested

Positive for WNV.sE/ELISA positivePositive for WNV.sE/VNT positiveMIA identification/
VNT identification

WNVSe = 99.0% 
(100/101)  
Sp = 98.1% 
(102/104)(1)
Se = 100.0% 
(99/99)
Se = 100.0% 
(96/96)
207 sera from Pakistan (68) + Madagascar (35) +
France (104)
JEVSe = 100.0% 
(88/88)(2)
Sp = 100.0% 
(11/11)
Se = 93.8% 
(90/96)  
Sp = 80.0% 
(4/5)(3)
Se = 90.1% 
(82/91)(4)
101 sera from Japan

Positive for WNV.sE/ELISA positivePositive for TBEV.EDIII/VNT positiveMIA identification/
VNT identification

TBEVSe = 100.0% 
(59/59)(5)
Sp = 100.0% 
(12/12)
Se = 98.4% 
(61/62)   
Sp = 100.0%  
(12/12)
Se = 96.8% 
(60/62)(6)
Sp = 100.0% 
(12/12)
74 sera from Austria

Same thresholds as in Table 3.
Positive for TBEV when the TBEV.EDIII MFI > 61.
(1)Two samples from the Camargue, France, were found positive by MIA but negative by ELISA.
(2)Two samples found doubtful by ELISA and positive by MIA were not taken into account for Se calculation (ELISA/MIA).
(3)One sample was found doubtful by ELISA, slightly positive by MIA with the WNV.sE bead, and negative by MNT.
(4)Three samples were identified as positive for WNV by MIA. One was also positive for WN by MNT while the other two were positive for an undetermined flavivirus.
(5)Three samples found doubtful by ELISA and positive () or negative () by MIA were not taken into account for Se calculation (ELISA/MIA).
(6)One undetermined sample by MIA due to TBEV.EDIII and WNV.EDIII reacting beads.