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BioMed Research International
Volume 2015, Article ID 746092, 10 pages
Research Article

Nucleofection of Rat Pheochromocytoma PC-12 Cells with Human Mutated Beta-Amyloid Precursor Protein Gene (APP-sw) Leads to Reduced Viability, Autophagy-Like Process, and Increased Expression and Secretion of Beta Amyloid

1Electron Microscopy Platform, Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warsaw, Poland
2Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776 Warsaw, Poland

Received 18 July 2014; Accepted 6 October 2014

Academic Editor: Patrycja Pawlikowska

Copyright © 2015 Beata Pająk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: The Vector Map of pCMV6-AC-GFP.

Figure S2: Phase-contrast and fluorescent views showing the phenotype of GFP vector (G), or GFP vector + APP-wt (W), or GFP vector + APP-sw (S) nucleofected PC-12 cells (24 hours after nucleofection). Bars represent 100 μm.

Figure S3: Analysis of PCR products.

Figure S4: Bar charts (means + SEM) represent cell viability (NR assay) expressed as % of control (untreated PC-12 cells nucleofected with GFP, or GFP + APP-wt, or GFP + APP-sw). Different lower case letters indicate statistically significant differences between means (P < 0.05).

Figure S5: Analysis of protein expression in the “flux” experiment additionally treated with rapamycin (1 μM) or chloroquine (30 μM) for the last hour of experiment.

  1. Supplementary Material