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BioMed Research International
Volume 2015, Article ID 859456, 9 pages
http://dx.doi.org/10.1155/2015/859456
Research Article

Microstereolithography-Based Fabrication of Anatomically Shaped Beta-Tricalcium Phosphate Scaffolds for Bone Tissue Engineering

1Department of Orthopaedic Surgery, Sino-Russian Institute of Hard Tissue Development and Regeneration, Harbin Medical University, Nangang, Harbin 150086, China
2Department of Bioengineering, University of Tokyo, Bunkyō, Tokyo 113-8656, Japan
3Department of Mechanical Engineering, Tokyo Denki University, Adachi, Tokyo 101-8457, Japan
4Center for Disease Biology and Integrative Medicine, University of Tokyo, Bunkyō, Tokyo 113-8656, Japan

Received 20 April 2015; Revised 3 August 2015; Accepted 3 August 2015

Academic Editor: Vladimir S. Komlev

Copyright © 2015 Dajiang Du et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Porous ceramic scaffolds with shapes matching the bone defects may result in more efficient grafting and healing than the ones with simple geometries. Using computer-assisted microstereolithography (MSTL), we have developed a novel gelcasting indirect MSTL technology and successfully fabricated two scaffolds according to CT images of rabbit femur. Negative resin molds with outer 3D dimensions conforming to the femur and an internal structure consisting of stacked meshes with uniform interconnecting struts, 0.5 mm in diameter, were fabricated by MSTL. The second mold type was designed for cortical bone formation. A ceramic slurry of beta-tricalcium phosphate (-TCP) with room temperature vulcanization (RTV) silicone as binder was cast into the molds. After the RTV silicone was completely cured, the composite was sintered at 1500°C for 5 h. Both gross anatomical shape and the interpenetrating internal network were preserved after sintering. Even cortical structure could be introduced into the customized scaffolds, which resulted in enhanced strength. Biocompatibility was confirmed by vital staining of rabbit bone marrow mesenchymal stromal cells cultured on the customized scaffolds for 5 days. This fabrication method could be useful for constructing bone substitutes specifically designed according to local anatomical defects.