Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

Figure 3

Mesenchymal stem cells (MSCs) under 7 days of unstrained in general medium (a), strain-stimulated in general medium (b), and unstrained in osteogenic medium (c), strain-stimulated in osteogenic medium (d). I-collagen protein levels of expression in cells were detected by immunocytochemical stain. I-collagen protein staining in the cytoplasm was stronger in the experimental groups (strain + osteogenic medium) at 7 days than in the control groups (general medium or osteogenic medium). Stressed cells were oriented perpendicularly to the strain axis, whereas, in the control group (unstrained), cells were randomly oriented.

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